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ELA3A Antibody (3G4)

Images

 
Western Blot: ELA3A Antibody (3G4) [H00010136-M02] - ELA3A monoclonal antibody (M02), clone 3G4. Analysis of ELA3A expression in human pancreas.
Immunohistochemistry-Paraffin: ELA3A Antibody (3G4) [H00010136-M02] - Analysis of monoclonal antibody to ELA3A on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Sandwich ELISA: ELA3A Antibody (3G4) [H00010136-M02] - Detection limit for recombinant GST tagged ELA3A is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
3G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ELA3A Antibody (3G4) Summary

Immunogen
ELA3A (AAH07028, 16 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGYGPPSSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSGFGCNFIWKPTVFTRVSAFIDWIEETIASH
Specificity
ELA3A - elastase 3A, pancreatic
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CELA3A
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ELA3A Antibody (3G4)

  • chymotrypsin-like elastase family member 3A
  • chymotrypsin-like elastase family, member 3A
  • EC 3.4.21
  • EC 3.4.21.70
  • ELA3A
  • ELA3elastase 1
  • elastase 3A, pancreatic (protease E)
  • elastase 3A, pancreatic
  • Elastase IIIA
  • elastase-3A
  • Protease E

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CELA3A
Entrez
Uniprot