![Western Blot: eIF5A2 Antibody [NBP1-85943] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Western-Blot-NBP1-85943-img0006.jpg "Western Blot: eIF5A2 Antibody [NBP1-85943] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: IKRNDYQLICIQDGYLSLLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EIF5A2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC reported in scientific literature (PMID: 24484057). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Immunohistochemistry eIF5A Antibody [Unconjugated]](https://images.novusbio.com/images2/eIF5A_AF7558_Immunohistochemistry_22678.jpg)
![Immunohistochemistry eIF5A Antibody [Unconjugated]](https://images.novusbio.com/images2/eIF5A_AF7558_Immunohistochemistry_22679.jpg)
![Immunohistochemistry eIF5A Antibody [Unconjugated]](https://images.novusbio.com/images2/eIF5A_AF7558_Immunohistochemistry_22682.jpg)
| Publication using NBP1-85943 | Applications | Species |
|---|---|---|
| Murata Y, Minami Y, Iwakawa R et al. ECT2 amplification and overexpression as a new prognostic biomarker for early-stage lung adenocarcinoma. Cancer Sci 2014-04-01 [PMID: 24484057] (IF/IHC, Human) | IF/IHC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for eIF5A2 Antibody (NBP1-85943)Find related products by research area.
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| Gene Symbol | EIF5A2 |
![Immunocytochemistry/Immunofluorescence: eIF5A2 Antibody [NBP1-85943] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85943-img0007.jpg "Immunocytochemistry/Immunofluorescence: eIF5A2 Antibody [NBP1-85943] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human kidney shows moderate positivity in nuclear membrane in cells in tubules.](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-85943-img0012.jpg "Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human kidney shows moderate positivity in nuclear membrane in cells in tubules.")
![Western Blot: eIF5A2 Antibody [NBP1-85943] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-182](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Western-Blot-NBP1-85943-img0005.jpg "Western Blot: eIF5A2 Antibody [NBP1-85943] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-182")
![Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells and a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-85943-img0010.jpg "Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells and a subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human cerebellum shows strong positivity in nuclear membrane in all layers.](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-85943-img0011.jpg "Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human cerebellum shows strong positivity in nuclear membrane in all layers.")
![Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human stomach shows moderate positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/eIF5A2-Antibody-Immunohistochemistry-Paraffin-NBP1-85943-img0013.jpg "Immunohistochemistry-Paraffin: eIF5A2 Antibody [NBP1-85943] - Staining of human stomach shows moderate positivity in nuclear membrane in glandular cells.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")