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Recombinant Mouse EGF Protein

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SDS-Page: Recombinant Mouse EGF Protein [NBP2-35176]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Mouse EGF Protein Summary

Description
A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF, including 3 intramolecular disulfide-bonds Source: E. coli

Uniprot ID: P01132

Amino Acid Sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Details of Functionality
EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
EGF
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of EGF as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
6.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse EGF Protein

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor (EGF) is the founding member of the EGF family of growth factors. Mature EGF is generated by proteolytic release of a juxtamembrane domain within the transmembrane EGF precursor. It is present in a wide variety of body fluids. EGF binds the receptor ErbB1 and induces signaling through ErbB1 homodimers or heterodimers with ErbB2. It plays a key role in epithelial morphogenesis, angiogenesis, and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol EGF
Uniprot