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Eg5 Antibody

Images

 
Western Blot: Eg5 Antibody [NBP2-58929] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NBP2-58929] - Staining of human cell line RH-30 shows localization to cytosol.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Eg5 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF11
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Eg5 Recombinant Protein Antigen (NBP2-58929PEP)

Reactivity Notes

Mouse 88%, Rat 83%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Eg5 Antibody

  • Eg5
  • HKSP
  • KIF11
  • kinesin family member 11
  • kinesin-like 1
  • Kinesin-like protein 1
  • kinesin-like protein KIF11
  • Kinesin-like spindle protein HKSP
  • Kinesin-related motor protein Eg5
  • KNSL1
  • KNSL1TRIP-5
  • thyroid receptor interacting protein 5
  • Thyroid receptor-interacting protein 5
  • TR-interacting protein 5
  • TRIP5
  • TRIP-5
  • TRIP5EG5

Background

Eg5 is a mitotic motor protein required for chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Eg-5 is therefore crucial for proper chromosome segregation during cell division. Eg 5 antibodies are useful tools for cell cycle studies and research on mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KIF11