![Western Blot: EFHD2 Antibody [NB100-1477] - Staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/EFHD2-Antibody-Western-Blot-NB100-1477-img0014.jpg "Western Blot: EFHD2 Antibody [NB100-1477] - Staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence CAAFKELQSTFK corresponding to C-Terminus according to NP_077305.2. |
| Specificity | This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz. |
| Predicted Species | Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | EFHD2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 30 kDa band observed in human tonsil, thymus and lymph node and in mouse thymus and lymph node lysates (calculated MW of 26.7 kDa band according to NP_077305.2). IHC-P: Human tonsil shows membranous staining in selected cells. |
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| Theoretical MW | 27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | mice | 04/27/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for EFHD2 Antibody (NB100-1477)Find related products by research area.
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![Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477] - Analysis of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/EFHD2-Antibody-Immunohistochemistry-Paraffin-NB100-1477-img0012.jpg "Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477] - Analysis of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
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