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DPH4 Antibody

Images

 
Western Blot: DPH4 Antibody [NBP1-87969] - Analysis in control (vector only transfected HEK293T lysate) and DNAJC24 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: DPH4 Antibody [NBP1-87969] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: DPH4 Antibody [NBP1-87969] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DPH4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MAVEQMPKKDWYSILGADPSANISDLKQKYQKLILMYHPDKQSTDVPAGTVEECVQKFIEIDQAWK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJC24
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DPH4 Protein (NBP1-87969PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DPH4 Antibody

  • CSL-type zinc finger-containing protein 3
  • DnaJ (Hsp40) homolog, subfamily C, member 24
  • dnaJ homolog subfamily C member 24
  • DPH4 homolog
  • DPH4, JJJ3 homolog (S. cerevisiae)
  • DPH4, JJJ3 homolog
  • DPH41700030A21Rik
  • JJJ3
  • S. cerevisiae)
  • ZCSL3
  • zinc finger, CSL domain containing 3
  • zinc finger, CSL-type containing 3

Background

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylationand inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved insynthesis of diphthamide in EEF2 (Liu et al., 2004 (PubMed 15485916)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DNAJC24