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DIMT1L Antibody

Images

 
Western Blot: DIMT1L Antibody [NBP2-30659] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: DIMT1L Antibody [NBP2-30659] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & cytosol.
Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659] - Staining of human squamous epithelia shows weak positivity in nucleoli in squamous epithelial cells.
Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659] - Staining of human hippocampus shows moderate nucleolar positivity in neurons.
Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659] - Staining of human cerebral cortex shows weak nucleoli positivity in neurons.
Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659] - Staining of human endometrium shows very weak positivity in nucleoli in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DIMT1L Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCV
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIMT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DIMT1L Protein (NBP2-30659PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DIMT1L Antibody

  • 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase
  • DIM1 dimethyladenosine transferase 1-like (S. cerevisiae)
  • DIM1 dimethyladenosine transferase 1-like
  • DIM1
  • DIMT1dimethyladenosine transferase
  • EC 2.1.1
  • EC 2.1.1.14
  • EC 2.1.1.183,18S rRNA dimethylase
  • HSA9761
  • HUSSY5
  • N'-adenosyl(rRNA) dimethyltransferase
  • probable dimethyladenosine transferase
  • S-adenosylmethionine-6-N'

Background

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DIMT1L Antibody (NBP2-30659) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DIMT1
Uniprot