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Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI6G7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70569AF350
Alexa Fluor 405
NBP2-70569AF405
Alexa Fluor 488
NBP2-70569AF488
Alexa Fluor 532
NBP2-70569AF532
Alexa Fluor 594
NBP2-70569AF594
Alexa Fluor 647
NBP2-70569AF647
Alexa Fluor 700
NBP2-70569AF700
Alexa Fluor 750
NBP2-70569AF750
Allophycocyanin
NBP2-70569APC
Biotin
NBP2-70569B
CoraFluor 1
NBP2-70569CL1
DyLight 350
NBP2-70569UV
DyLight 405
NBP2-70569V
DyLight 488
NBP2-70569G
DyLight 550
NBP2-70569R
DyLight 594
NBP2-70569DL594
DyLight 680
NBP2-70569FR
DyLight 755
NBP2-70569IR
FITC
NBP2-70569F
HRP
NBP2-70569H
Janelia Fluor 525
NBP2-70569JF525
Janelia Fluor 549
NBP2-70569JF549
Janelia Fluor 646
NBP2-70569JF646
Janelia Fluor 669
NBP2-70569JF669
PE
NBP2-70569PE
PerCP
NBP2-70569PCP
Unconjugated
NBP1-47727
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47727
Azide and BSA Free
NBP2-70569
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Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [DyLight 650] Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human Dihydrofolate Reductase/DHFR expression vector.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DHFR
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Dihydrofolate Reductase/DHFR Antibody (OTI6G7) [DyLight 650]

  • DHFR
  • DHFRP1
  • Dihydrofolate Reductase
  • DYR
  • EC 1.5.1.3

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DHFR