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Dermcidin Antibody (G-81)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
G-81
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Dermcidin Antibody (G-81) Summary

Immunogen
Fractioned sweat peptides in complete Freunds adjuvant
Specificity
The monoclonal antibody G-81 was produced against a sweat-specific antigen. It reacts to sweat but not to other body fluids (e.g. serum, saliva, semen, milk, urine and tears) in ELISA. The N-terminal amino acid sequence (18 amino acids residues) is identical to a part of human cachexia-associated protein and of a survival-promoting peptide for neuronal cells. Besides this, the sequence is identical to the C-terminal peptide (amino acids 62-79) of DCD. The monoclonal antibody G81 can be used as a specific marker for eccrine glands in the human skin.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
DCD
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
NBP1-49620 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Tissue culture supernatant
Preservative
0.02% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Dermcidin Antibody (G-81)

  • AIDDdiffusible survival/evasion peptide
  • DCD-1
  • dermcidin
  • DSEPpreproteolysin
  • HCAP
  • MGC71930
  • PIF
  • Preproteolysin
  • proteolysis inducing factor
  • survival promoting peptide

Background

The monoclonal antibody G-81 recognizes a fragment of human dermcidin (DCD). The antibiotic peptide dermcidin (110 amino acids) is specifically and constitutively expressed in the sweat glands. DCD is localized to the dark mucous cells of normal eccrine sweat glands. DCD is normally expressed in eccrine sweat glands and is expressed in cutaneous mixed tumours. DCD-immunopositive cells are expressed in the inner cells of the tubular and ductal structures. The monoclonal antibody G-81 is useful as a immunohistochemical tool to find the tumour cells that differentiate towards eccrine secretory cells in cutaneous mixed tumours. Furthermore, the antibody can be used for the detection of biochemical and histochemical changes of the sweat gland in the healing process of wound.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dermcidin Antibody (NBP1-49620)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IF/IHC.


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ICC/IF
(2)
IF/IHC
(1)
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Human
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dermcidin Antibody (NBP1-49620) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DCD
Entrez
Uniprot