Home » DEK » DEK Antibodies » DEK Antibody (715524) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

DEK Antibody (715524) [Unconjugated]

 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and OVCAR-3 human ovarian carcinoma cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 30 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB7020) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and OVCAR-3 human ovarian carcinoma cell line. ...read more
DEK was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB7020) at 5 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
715524
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7020-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

DEK Antibody (715524) [Unconjugated] Summary

Immunogen
Human embryonic kidney cell line HEK293EBNA-derived recombinant human DEK
Ser2-Ser375
Accession # P35659
Specificity
Detects human DEK in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DEK
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEK Antibody (715524) [Unconjugated]

  • D6S231EDEK oncogene (DNA binding)
  • DEK oncogene
  • DEK
  • protein DEK

Background

The DEK oncogene is an approximately 45 kDa phosphoprotein with pleiotropic activities. It is a chromatin remodeling factor, a transcriptional coactivator and corepressor, a component of RNA spliceosomes, and a promoter of DNA repair during apoptosis. DEK is upregulated in many cancers and promotes epithelial cell transformation. It functions as a proinflammatory factor when secreted by activated macrophages. It is an autoantigen in juvenile arthritis and lupus and a target of chromosomal translocation with the CAN gene in acute myelogenous leukemia. DEK contains one SAP domain (aa 149-183), one nuclear localization sequence (aa 205-221), and a DNA-binding C-terminal domain (aa 321-375). Within aa 292-375, human DEK shares 95% aa sequence identity with mouse and rat DEK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CDK5 Activator 1
Antibody
NBP3-13279
Immunocytochemistry/ Immunofluorescence CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Immunohistochemistry-Paraffin CDK5 Activator 1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
TPT1/TCTP Antibody
(2A3)
H00007178-M06
Western Blot TPT1/TCTP Antibody (2A3)
Western Blot TPT1/TCTP Antibody (2A3)
Western Blot TPT1/TCTP Antibody (2A3)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, WB
Tmp21/p23 Antibody
- BSA Free
NB110-57586
Knockdown Validated Tmp21/p23 Antibody - BSA Free
Western Blot Tmp21/p23 Antibody - BSA Free
Immunohistochemistry Tmp21/p23 Antibody - BSA Free
Species: Bv, Ch, Hu, Mu, Pm, Rb, Rt
Applications: IHC, IHC-P, KD, WB
RRAS Antibody
NB100-56161
Immunohistochemistry-Paraffin RRAS Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
p23/PTGES3
Antibody (JJ3)
NB300-576
Immunohistochemistry-Paraffin p23/PTGES3 Antibody (JJ3)
Immunohistochemistry-Paraffin p23/PTGES3 Antibody (JJ3)
Immunohistochemistry-Paraffin p23/PTGES3 Antibody (JJ3)
(8 Publications)
Species: Ch, Gp, Hu, Mu, Pm, Rb, Rt, Xp
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
c-Abl Antibody
[Unconjugated]
AF5414
Western Blot c-Abl Antibody [Unconjugated]
Simple Western c-Abl Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: Simple Western, WB
E2F3 Antibody
NBP1-80295
Western Blot E2F3 Antibody
Western Blot E2F3 Antibody
Western Blot E2F3 Antibody
(1 Publication)
Species: Hu, Mu
Applications: WB
PCAF Antibody
(5E11)
H00008850-M04
Immunocytochemistry/ Immunofluorescence PCAF Antibody (5E11)
Sandwich ELISA PCAF Antibody (5E11)
Western Blot PCAF Antibody (5E11)
(1 Review)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
BCR Antibody
AF5129
Western Blot BCR Antibody
(1 Publication)
Species: Hu
Applications: WB
MeCP2 Antibody
NB100-56326
Western Blot MeCP2 Antibody
Immunocytochemistry/ Immunofluorescence MeCP2 Antibody
Immunohistochemistry-Paraffin MeCP2 Antibody
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NPM1 Antibody -
BSA Free
NB110-61646
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Western Blot NPM1 Antibody - BSA Free
(2 Reviews)
(4 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
SRRM1 Antibody
NBP2-13381
Immunohistochemistry-Paraffin SRRM1 Antibody
Simple Western SRRM1 Antibody
Simple Western SRRM1 Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot HIST1H4F Overexpression Lysate
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2) - BSA Free
NBP2-52486
ELISA H4/n Antibody (3E7H2) - BSA Free
Western Blot H4/n Antibody (3E7H2) - BSA Free
Flow Cytometry H4/n Antibody (3E7H2) - BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Aly Antibody
(JE36-45)
NBP3-32033
Western Blot Aly Antibody (JE36-45)
Immunohistochemistry Aly Antibody (JE36-45)
Immunocytochemistry/ Immunofluorescence Aly Antibody (JE36-45)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NUP214 Antibody
NB100-2244
Western Blot NUP214 Antibody
Immunohistochemistry-Paraffin NUP214 Antibody
Immunohistochemistry-Paraffin NUP214 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, IP, WB
Peroxiredoxin 6
Antibody (3A10-2A11)
H00009588-M01
ELISA Peroxiredoxin 6 Antibody (3A10-2A11)
Western Blot Peroxiredoxin 6 Antibody (3A10-2A11)
Western Blot Peroxiredoxin 6 Antibody (3A10-2A11)
(12 Publications)
Species: Bv, Hu
Applications: DB, ELISA, WB
RUNX1/CBFA2
Antibody
NBP1-89105
Immunocytochemistry/ Immunofluorescence RUNX1/CBFA2 Antibody
Immunohistochemistry-Paraffin RUNX1/CBFA2 Antibody
Immunohistochemistry-Paraffin RUNX1/CBFA2 Antibody
(1 Review)
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
RB1 Antibody
(607121) [Unconjugate
MAB6495
Western Blot RB1 Antibody (607121) [Unconjugated]
Immunocytochemistry RB1 Antibody (607121) [Unconjugated]
Simple Western RB1 Antibody (607121) [Unconjugated]
(5 Publications)
Species: Hu
Applications: ICC, Simple Western, WB

Publications for DEK Antibody (MAB7020) (0)

There are no publications for DEK Antibody (MAB7020).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DEK Antibody (MAB7020) (0)

There are no reviews for DEK Antibody (MAB7020). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DEK Antibody (MAB7020) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DEK Products

Blogs on DEK

There are no specific blogs for DEK, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DEK Antibody (715524) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol DEK
Uniprot