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DEK Antibody (715511) [Unconjugated]

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DEK was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DEK was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
DEK was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 5 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
715511
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB70201-SP
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DEK Antibody (715511) [Unconjugated] Summary

Immunogen
Human human embryonic kidney cell line HEK293EBNA-derived recombinant human DEK
Val282-Ser375
Accession # P35659
Specificity
Detects human DEK in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DEK
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEK Antibody (715511) [Unconjugated]

  • D6S231EDEK oncogene (DNA binding)
  • DEK oncogene
  • DEK
  • protein DEK

Background

The DEK oncogene is an approximately 45 kDa phosphoprotein with pleiotropic activities. It is a chromatin remodeling factor, a transcriptional coactivator and corepressor, a component of RNA spliceosomes, and a promoter of DNA repair during apoptosis. DEK is upregulated in many cancers and promotes epithelial cell transformation. It functions as a proinflammatory factor when secreted by activated macrophages. It is an autoantigen in juvenile arthritis and lupus and a target of chromosomal translocation with the CAN gene in acute myelogenous leukemia. DEK contains one SAP domain (aa 149-183), one nuclear localization sequence (aa 205-221), and a DNA-binding C-terminal domain (aa 321-375). Within aa 292-375, human DEK shares 95% aa sequence identity with mouse and rat DEK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DEK
Uniprot