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Human Defensin alpha 5 ELISA Kit (Colorimetric)

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ELISA: Human Defensin alpha 5 ELISA Kit (Colorimetric) [NBP3-24601] - Samples were spiked with high concentrations of Human Defensin alpha 5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Defensin alpha 5 ELISA Kit (Colorimetric) [NBP3-24601] - Samples were spiked with high concentrations of Human Defensin alpha 5 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Defensin alpha 5 ELISA Kit (Colorimetric) [NBP3-24601] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Human Defensin alpha 5 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Defensin Alpha 5 in samples. No significant cross-reactivity or interference between Human Defensin Alpha 5 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.73
Intra-Assay
%CV < 5.62
Spike Recovery
87 - 105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
DEFA5

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Defensin alpha 5 ELISA Kit (Colorimetric)

  • alpha-Defensin 5
  • DEF5
  • DEF5defensin, alpha 5, preproprotein
  • DEFA5
  • defensin 5
  • Defensin alpha 5
  • Defensin, alpha 5
  • defensin, alpha 5, Paneth cell-specific
  • defensin-5
  • HD-5
  • MGC129728

Background

Defensin alpha 5 (DEFA5) is primarily responsible for host defense. DEFA5 has shown antimicrobial activity and is thought to kill microbes by permeabilizing their plasma membrane. This protein is highly expressed in the secretory granules of Paneth cells of the ileum.

Defensin alpha 5 antibodies are useful tools for bacterial defense studies and human immunology research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Human Defensin alpha 5 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol DEFA5