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DC8 Antibody

Images

 
Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575] - Detection of human DC8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DC8 (NB100-2575) used ...read more
Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-DC8 used at a dilution of 1:250.
Immunohistochemistry: DC8 Antibody [NB100-2575] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DC8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: DC8 Antibody [NB100-2575] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DC8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: DC8 Antibody [NB100-2575] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-2575 used for IP at 3 mcg/mg lysate. DC8 was also immunoprecipitated by rabbit anti-DC8 ...read more
Independent Antibodies: DC8 Antibody [NB100-2575] - Detection of human DC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

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DC8 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human DC8 (C1orf48) using the numbering given in entry NP_056286.2 (GeneID 25936 ).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NSL1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Control
HeLa Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DC8 Antibody

  • C1orf48
  • chromosome 1 open reading frame 48
  • DC8
  • DKFZp566O1646
  • kinetochore-associated protein NSL1 homolog
  • MIS14
  • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae)

Background

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. It is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NSL1
Entrez
Uniprot