![Western Blot: DAZL Antibody (DAZL/4253) [NBP3-07840] - Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).](http://images.novusbio.com/fullsize/DAZL-Antibody-DAZL-4253-Western-Blot-NBP3-07840-img0002.jpg "Western Blot: DAZL Antibody (DAZL/4253) [NBP3-07840] - Western blot Analysis testis tissue lysate using DAZL Mouse Monoclonal Antibody (DAZL/4253).")
| Reactivity | Hu, Mu, Rt, Pm-CmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | DAZL/4253 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08742) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV. (Uniprot: Q92904) |
| Localization | Cytoplasmic |
| Specificity | Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DAZL |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) [NBP3-07840] - Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).](http://images.novusbio.com/fullsize/DAZL-Antibody-DAZL-4253-Immunohistochemistry-Paraffin-NBP3-07840-img0001.jpg "Immunohistochemistry-Paraffin: DAZL Antibody (DAZL/4253) [NBP3-07840] - Formalin-fixed, paraffin-embedded human testis stained with DAZL Mouse Monoclonal Antibody (DAZL/4253).")
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