![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Western-Blot-NBP2-22194-img0008.jpg "Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 2C2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Cytokeratin 5 expressed in E. Coli. |
| Localization | Cytoplasm |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | This antibody is Cytof ready. |
|
| Theoretical MW | 62.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
IHC-Fr | Human | 12/27/2019 |
Summary
Comments
|
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 5 Antibody (NBP2-22194)Find related products by research area.
|
|
Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You? By Jennifer Jones, M.S.Spheroids and organoids are two words that, like “butter” and “margarine”, are often referred to interchangeably but have distinct meanings. The progression and adopt... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT5 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunohistochemistry-Paraffin-NBP2-22194-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.")
![Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - An intracellular stain was performed on HeLa cells with Cytokeratin 5 Antibody (2C2) NBP2-22194APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Flow-Cytometry-NBP2-22194-img0009.jpg "Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - An intracellular stain was performed on HeLa cells with Cytokeratin 5 Antibody (2C2) NBP2-22194APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.")
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mAb against human Cytokeratin 5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Western-Blot-NBP2-22194-img0007.jpg "Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mAb against human Cytokeratin 5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells using Cytokeratin 5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells using Cytokeratin 5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of breast cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunohistochemistry-Paraffin-NBP2-22194-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of breast cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.")
![Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of HeLa cells using Cytokeratin 5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Flow-Cytometry-NBP2-22194-img0001.jpg "Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of HeLa cells using Cytokeratin 5 mouse mAb (green) and negative control (red).")
![ELISA: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-ELISA-NBP2-22194-img0006.jpg "ELISA: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)")
![Immunohistochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunohistochemistry_16919.jpg)
![Immunocytochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunocytochemistry__Immunofluorescence_16973.jpg)
![Immunocytochemistry/ Immunofluorescence p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images/af1916_human-p63-tp73l-affinity-purified-polyclonal-ab-41202412401237.jpg)
![Immunocytochemistry CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Immunocytochemistry__Immunofluorescence_17000.jpg)
![Western Blot CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Western_Blot_11997.jpg)
![Immunocytochemistry CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Immunocytochemistry_12002.jpg)
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Immunocytochemistry Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Immunocytochemistry__Immunofluorescence_20674.jpg)
![Immunohistochemistry Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Immunohistochemistry_20779.jpg)
![Western Blot Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Western_Blot_5715.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
