![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/4866) [NBP3-07646] - Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-KRT5-6-4866-Immunohistochemistry-Paraffin-NBP3-07646-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/4866) [NBP3-07646] - Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | KRT5.6/4866 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08683) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human Cytokeratin 5/6 protein |
| Localization | Cytoplasmic |
| Marker | Basal, Myoepithelial & Mesothelial Cell Marker |
| Specificity | It recognizes polypeptides of 58kDa and 56kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma. |
| Isotype | IgG1 Kappa/IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT5 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/4866) [NBP3-07646] - Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).](http://images.novusbio.com/fullsize/Cytokeratin-5-6-Antibody-KRT5-6-4866-Immunohistochemistry-Paraffin-NBP3-07646-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/4866) [NBP3-07646] - Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).")
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