![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0004.jpg "Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QDRKLDENANVQLSD corresponding to internal region according to Uniprot Mouse NP_001129531.1. |
| Specificity | This antibody is not expected to cross-react with the similar P450 1A2 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CYP1A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Approx. 55 kDa band observed in human and rat lung lysates (calculated MW of 59.2 kDa band according to mouse NP_001129531.1 and 59.4 kDa band according to rat NP_036672.). |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-97762 | Applications | Species |
|---|---|---|
| Sulem P, Gudbjartsson DF, Geller F et al. Sequence variants at CYP1A1-CYP1A2 and AHR associate with coffee consumption. Hum Mol Genet 2011-05-15 [PMID: 21357676] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome P450 1A1 Antibody (NBP1-97762)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CYP1A1 |
| Entrez | |
| Uniprot |
|
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1 Antibody [NBP1-97762] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97762-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1 Antibody [NBP1-97762] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0006.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.")
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Rat Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0002.jpg "Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Rat Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining. Antibody at 1:500.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0005.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining. Antibody at 1:500.")
