![Western Blot: Cytochrome c Antibody (CYCS/3128R) - Azide and BSA Free [NBP3-08598] - Western Blot Analysis of human heart tissue lysate using Cytochrome c Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-3128R-Azide-and-BSA-Free-Western-Blot-NBP3-08598-img0004.jpg "Western Blot: Cytochrome c Antibody (CYCS/3128R) - Azide and BSA Free [NBP3-08598] - Western Blot Analysis of human heart tissue lysate using Cytochrome c Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).")
| Reactivity | Hu, Mu, Rt, Am, Ca, Dr, Eq, PgnSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | CYCS/3128R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07619). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant full-length human Cytochrome c protein (Uniprot: P99999) |
| Epitope | aa 93-104 |
| Localization | Cytoplasmic |
| Marker | Mitochondrial Marker |
| Specificity | It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This monoclonal antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CYCS |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 1mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytochrome c Antibody (NBP3-08598)Find related products by research area.
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Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to... Read full blog post. |
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The use of apoptosis antibodies and controls in cell death research Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance. Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha... Read full blog post. |
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Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co... Read full blog post. |
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Cytochrome C - a mediator of apoptosis Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain. Additionally, cytochrome c has also been identified as a player in programmed cell death (apop... Read full blog post. |
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Cytochrome C in Apoptosis, Immune Response and Cancer Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio... Read full blog post. |
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Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS) Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese... Read full blog post. |
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Bax Research Gives New Insight into Oxidative Apoptosis Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers... Read full blog post. |
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The Role of the Caspase 3 Antibody in Apoptosis Research The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are... Read full blog post. |
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| Gene Symbol | CYCS |
![Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (CYCS/3128R) - Azide and BSA Free [NBP3-08598] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cytochrome c Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by goat anti-rabbit IgG-CF488 (Green)](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-3128R-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08598-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (CYCS/3128R) - Azide and BSA Free [NBP3-08598] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cytochrome c Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by goat anti-rabbit IgG-CF488 (Green)")
![Immunohistochemistry-Paraffin: Cytochrome c Antibody (CYCS/3128R) - Azide and BSA Free [NBP3-08598] - Formalin-fixed, paraffin-embedded human Liver stained with Cytochrome c Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CYCS-3128R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08598-img0003.jpg "Immunohistochemistry-Paraffin: Cytochrome c Antibody (CYCS/3128R) - Azide and BSA Free [NBP3-08598] - Formalin-fixed, paraffin-embedded human Liver stained with Cytochrome c Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).")
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