I want to carry out a research using cystatin c to determine GFR in sickle cell anemia patients. Can pin prick sample be used? What is the procedure to carry out the test?

Name: Human, Monkey Cystatin C ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA0022
Price: 1029 USD

Question

Accepted Answer

The cystatin C ELISA kits we sell are manufactured by two companies, Abnova and Biosensis, and distributed by us here at Novus Biologicals. I contacted both companies to ask them your question. I have now heard back from Biosensis, who produce the product catalog # BEK-2145-2P. Their answer was as follows: We haven't used our kit with such samples; however, a recent paper suggests that it could be possible. The paper used a different method than traditional ELISA, but if the samples were allowed to clot or collected in citrated tubes it certainly seems possible to use such samples, but the customer will have to investigate on their own the true effectiveness compared to traditional blood sample collection, for which the kit is tested. Here is a link to the paper examining capillary tube collection (https://academic.oup.com/clinchem/article/51/5/903/5629843) These people even have a patent on using the technology for the measure of cystatin C for human samples (http://helierscientific.co.uk/renalfunction.html) so that too might be an issue for your customer. Finally, this paper too probably should be reviewed by your customer; it is more modern and discusses a number of approaches and issues (https://journals.lww.com/cjasn/pages/articleviewer.aspx?year=2009&issue=11000&article=00029&type=Fulltext) Just to let you know that I have now heard from Abnova, the manufacturer of our cystatin C ELISA kits product catalog # KA0022 (protocol: http://www.abnova.com/protocol_pdf/KA0022.pdf) and catalog # KA2107 (protocol: http://www.abnova.com/protocol_pdf/KA2107.pdf). Their response was simply that neither of the kits have been tested on pin-prick blood samples, and so they are not sure whether these kits are suitable or not.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, PmSpecies Glossary
Applications	ELISA, NULL
Suitable Sample Type	Cerebrospinal Fluid, Plasma (Citrate, EDTA, Heparin), Serum, Urine
Standard Curve Range	200 to 10000 ng/mL
Sensitivity	0.25 ng/mL

Description	Quality control test: Standard curve. CST3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human cystatin C.
Standard Curve Range	200 to 10000 ng/mL
Sensitivity	0.25 ng/mL
Assay Type	Sandwich ELISA
Inter-Assay	CV% < 5.50%
Intra-Assay	CV% < 7.30%
Spike Recovery	90.80%
Sample Volume	100 uL (diluted)
Kit Type	ELISA Kit (Colorimetric)
Gene	CST3

Human, Monkey Cystatin C ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human, Monkey Cystatin C ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Notes

Alternate Names for Human, Monkey Cystatin C ELISA Kit (Colorimetric)

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Cystatin C ELISA Kit (KA0022)(1)

Reviews for Cystatin C ELISA Kit (KA0022) (0)

Product General Protocols

FAQs for Cystatin C ELISA Kit (KA0022). (Showing 1 - 1 of 1 FAQ).

Additional Cystatin C Products

Research Areas for Cystatin C ELISA Kit (KA0022)

Blogs on Cystatin C

Contact Information

Product PDFs

Review this Product

Bioinformatics