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Cyclin B1 Antibody (1C8)

Images

 
Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to Cyclin B1 on formalin-fixed paraffin-embedded human tonsil. Antigen retrieval was done enzymatically. Primary ...read more
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to CCNB1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 5 ug/ml.
ELISA: Cyclin B1 Antibody (1C8) [H00000891-M01] - Detection limit for recombinant GST tagged CCNB1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
1C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Cyclin B1 Antibody (1C8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CCNB1 (NP_114172, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLV
Marker
Mitosis Marker
Specificity
CCNB1 - cyclin B1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCNB1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.
Reviewed Applications
Read 1 Review rated 5
using
H00000891-M01 in the following applications:

Publications
Read Publication using H00000891-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cyclin B1 Antibody (1C8)

  • CCNB
  • CCNB1
  • Cyclin B1
  • G2/mitotic-specific cyclin B1
  • G2/mitotic-specific cyclin-B1

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00000891-M01
Species: Hu
Applications: WB, ELISA, IHC

Publications for Cyclin B1 Antibody (H00000891-M01)(1)

Review for Cyclin B1 Antibody (H00000891-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00000891-M01:
Filter by Applications
IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Human 12/26/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTonsil tissue
SpeciesHuman
Lot1C8

Comments

CommentsPFA fixed paraffin-embedded sections. Antigen retrieval was done enzymatically. Primary antibody dilution was 1:100.

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FAQs for Cyclin B1 Antibody (H00000891-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Verified Customer
12/26/2021
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CCNB1
Entrez
OMIM
Uniprot