Cyclin B1 Antibodies
We offer Cyclin B1 Antibodies for use in common research applications: ELISA, Flow Cytometry, ICC/IF (Negative), Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each Cyclin B1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Cyclin B1 Antibodies can be used in a variety of model species: Hamster, Human, Mouse, Rat. Use the list below to choose the Cyclin B1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Cyclin B1 polyclonal antibodies and browse our Cyclin B1 monoclonal antibody catalog.
Alternate Names for Cyclin B1 Antibodies
anti-Cyclin B1 antibody, anti-CCNB1 antibody, anti-CCNB antibody, anti-G2/mitotic-specific cyclin B1 antibody, anti-G2/mitotic-specific cyclin-B1 antibody
18 Results for "cyclin-b1" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunocytochemistry-Immunofluorescence-NBP2-44687-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Ovarian Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0002.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-CCNB1-1098-Immunohistochemistry-Paraffin-NBP2-44687-img0004.jpg)
![Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Western-Blot-MAB6000-img0002.jpg "Western Blot: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Western blot detection of Cyclin B1 (clone 2061A) catalog number MAB6000 at 0.05 ug/ml in HeLa and Jurkat cell lysates.")
![Immunohistochemistry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Cyclin B1 Monoclonal Antibody at 3 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Immunohistochemistry-MAB6000-img0003.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - An intracellular stain was performed on HeLa cells with Cyclin B1 Antibody [2061A] MAB6000F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0004.jpg)
![Flow Cytometry: Cyclin B1 Antibody (2061A) - Azide Free [MAB6000] - K562 human cell line was stained with Rabbit Anti-Human Cyclin B1 Monoclonal Antibody (Catalog # MAB6000, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-2061A-Azide-Free-Flow-Cytometry-MAB6000-img0001.jpg)
![Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Western-Blot-H00000891-M01-img0007.jpg "Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to Cyclin B1 on formalin-fixed paraffin-embedded human tonsil. Antigen retrieval was done enzymatically. Primary antibody dilution was 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Immunohistochemistry-Paraffin-H00000891-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to CCNB1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Immunohistochemistry-Paraffin-H00000891-M01-img0005.jpg)
![ELISA: Cyclin B1 Antibody (1C8) [H00000891-M01] - Detection limit for recombinant GST tagged CCNB1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-ELISA-H00000891-M01-img0008.jpg)
![Western Blot: Cyclin B1 Antibody [NBP2-56867] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Western-Blot-NBP2-56867-img0003.jpg "Western Blot: Cyclin B1 Antibody [NBP2-56867] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody [NBP2-56867] - Staining of human cell line A549 shows localization to cytosol.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56867-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867] - Staining in human testis and pancreas tissues using anti-CCNB1 antibody. Corresponding CCNB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0007.jpg)
![Immunohistochemistry: Cyclin B1 Antibody [NBP2-56867] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in subset of glandular cells.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-NBP2-56867-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 MAb (SPM619)followed by Goat anti-mouse IgG-CF488 (Green).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-SPM619-Immunocytochemistry-Immunofluorescence-NBP2-44688-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cyclin B1 MAb (SPM619)followed by Goat anti-mouse IgG-CF488 (Green).")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (SPM619) [NBP2-44688] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (SPM619)](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-SPM619-Immunohistochemistry-Paraffin-NBP2-44688-img0001.jpg)
![Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-V152-Flow-Cytometry-NBP3-07017-img0001.jpg "Flow Cytometry: Cyclin B1 Antibody (V152) [NBP3-07017] - Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5.0 ug/mL, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-5G6-Western-Blot-NBP1-51669-img0006.jpg "Western Blot: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-5G6-Immunocytochemistry-NBP1-51669-img0009.jpg)
![Flow Cytometry: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-5G6-Flow-Cytometry-NBP1-51669-img0007.jpg)
![Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Analysis of extracts of various cell lines, using Cyclin B1 antibody (NBP3-15626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1Q3E9-Western-Blot-NBP3-15626-img0003.jpg "Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Analysis of extracts of various cell lines, using Cyclin B1 antibody (NBP3-15626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Human colon carcinoma using [KO Validated] Cyclin B1 Rabbit mAb (NBP3-15626) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1Q3E9-Immunohistochemistry-Paraffin-NBP3-15626-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Western blot analysis of extracts from normal (control) and Cyclin B1 knockout (KO) HeLa cells, using Cyclin B1 antibody (NBP3-15626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1Q3E9-Knockout-Validated-NBP3-15626-img0001.jpg)
![Immunoprecipitation: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Analysis of 300ug extracts of HeLa cells using 3ug Cyclin B1 antibody (NBP3-15626). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (NBP3-15626) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1Q3E9-Immunoprecipitation-NBP3-15626-img0004.jpg)
![Western Blot: Cyclin B1 Antibody (RM281) [NBP2-61517] - Western Blot of Jurkat cell lysates using NBP2-61517 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-RM281-Western-Blot-NBP2-61517-img0004.jpg "Western Blot: Cyclin B1 Antibody (RM281) [NBP2-61517] - Western Blot of Jurkat cell lysates using NBP2-61517 at a 1:1000 dilution")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (RM281) [NBP2-61517] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using NBP2-61517 at a 1:1000 dilution.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-RM281-Immunohistochemistry-Paraffin-NBP2-61517-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (RM281) [NBP2-61517] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61517 at a 1:2000 dilution](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-RM281-Immunohistochemistry-Paraffin-NBP2-61517-img0002.jpg)