Detects mouse CXCL9/MIG in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with rhCXCL9 and 0.005% cross‑reactivity with rmMIP‑2, rrCINC‑1, rhGRO alpha , rhGRO beta and rhGRO gamma is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CXCL9/MIG Antibody [Biotin]
chemokine (C-X-C motif) ligand 9
CMK
crg-10
C-X-C motif chemokine 9
CXCL9
Gamma-interferon-induced monokine
Humig
MIG
MIGSmall-inducible cytokine B9
monokine induced by gamma interferon
SCYB9Monokine induced by interferon-gamma
Background
CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma . CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
Liao, F. et al. (1995) J. Exp. Med. 182:1301.
Vanguri, P. (1995) J. Neuroimmunol. 56:35.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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