![Western Blot: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Detection of CUG-BP1 in several cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Azide-and-BSA-Free-Western-Blot-NBP2-80684-img0001.jpg "Western Blot: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Detection of CUG-BP1 in several cell lysates. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Pm, RbSpecies Glossary |
| Applications | WB, Simple Western, Flow, GS, ICC/IF, IHC, IP |
| Clone | 3B1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Full-length human CUGBP1 [UniProt# Q92879] |
| Localization | Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CELF1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western Blot, a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CUGBP1/CELF1 Antibody (NBP2-80684)Find related products by research area.
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| Gene Symbol | CELF1 |
![Immunocytochemistry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80684-img0003.jpg "Immunocytochemistry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate")
![Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80684-img0004.jpg "Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80684-img0002.jpg "Immunocytochemistry/Immunofluorescence: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts. Image from the standard format of this antibody.")
![Simple Western: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Azide-and-BSA-Free-Simple-Western-NBP2-80684-img0005.jpg "Simple Western: CUGBP1/CELF1 Antibody (3B1) - Azide and BSA Free [NBP2-80684] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")