Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free

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Protein Array: Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free [NBP2-59601] - Analysis of Protein Array containing more than 19,000 containing more than 19,000 full-length human proteins. Z- and S- Score: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, MA, CyTOF-ready
Clone
2ba6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59600).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Human Creatine Kinase BB protein (Uniprot: P12277)
Localization
Cytoplasmic
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This monoclonal antibody recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CKB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Protein Array
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen Only): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.

Reactivity Notes

Predicted to react with Chimpanzee, Rhesus Monkey, Canine, Bovine, Mouse, Rat, Chicken, Zebrafish, Frog.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Creatine Kinase BB Antibody (2ba6) - Azide and BSA Free

  • B-CK
  • CKB
  • Creatine Kinase BB
  • Creatine Kinase Isoenzyme

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CKB