I need some information regarding the complement factor H antibodies. Catalogue no is H00003075-B01 and NBP1-33628. Does this antibody cross react with bovine Factor H?

Name: Complement Factor H Antibody
Brand: Novus Biologicals
SKU: NBP1-33628
Price: 449 USD

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Accepted Answer

I have carried out a blast to check the homology of the human protein Factor H with bovine Factor H. The % homology is 61%. Our lab only recommends use if the % homology is 85% or above, however if you would like to test this you will be eligible for our Innovators Reward Program.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Complement Factor H. The exact sequence is proprietary.
Localization	Secreted
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CFH
Purity	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry 1:100-1:1000 Immunohistochemistry-Paraffin 1:100-1:1000 Western Blot 1:500-1:10000
Theoretical MW	139 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7), 20% Glycerol
Preservative	0.01% Thimerosal
Purity	Antigen Affinity-purified

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