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Human Complement Component C9 ELISA Kit (Colorimetric)

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ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-82139] - Samples were spiked with high concentrations of Human Complement Component C9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-82139] - Samples were spiked with high concentrations of Human Complement Component C9 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-82139] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.47 - 30 ng/mL
Sensitivity
0.28 ng/mL

Order Details

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Human Complement Component C9 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Complement Component C9 in samples. No significant cross-reactivity or interference between Human Complement Component C9 and analogues was observed.
Standard Curve Range
0.47 - 30 ng/mL
Sensitivity
0.28 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 98% for serum; average = 102% for EDTA plasma; average = 100% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C9

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Human Complement Component C9 ELISA Kit (Colorimetric)

  • complement component 9
  • Complement Component C9

Background

C9 is synthesised in the liver and monocytes, and is a plasma protein consisting of a single polypeptide chain of molecular weight 71kDa. Normal plasma concentration is 60mg/L. C9 forms part of the membrane attack complex (MAC) the cytolytic terminal complex of the complement pathways. C9 binds to the membrane associated C5b-8, binding of C9 to C5b-8 leads to the circular polymerisation of 12-18 C9 molecules. This is the basis of the hydrophilic, protein-walled, trans-membrane channel formed by the MAC, which leads to cell lysis and destruction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Human Complement Component C9 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol C9