| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA(Det) |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant mouse C5a (R&D Systems, Catalog # 2150-C5) Asn679-Arg755 Accession # P06684 |
| Specificity | Detects mouse Complement Component C5a in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rhC5a, rhC3a, rmC3d and rhAlpha‑2 macroglobulin is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | Hc |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA Capture: Mouse Complement Component C5a Antibody (Catalog # MAB21501) ELISA Detection: Mouse Complement Component C5a Biotinylated Antibody (Catalog # BAF2150) Standard: Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) |
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| Readout System | ||
| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).
![Complement Component C5a Antibody [Biotin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Complement Component C5a Antibody [Biotin]")
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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