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Mouse Complement C5 ELISA Kit (Colorimetric)

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ELISA: Mouse Complement C5 ELISA Kit (Colorimetric) [NBP2-70049] - Samples were spiked with high concentrations of Mouse Complement C5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse Complement C5 ELISA Kit (Colorimetric) [NBP2-70049] - Samples were spiked with high concentrations of Mouse Complement C5 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Mouse Complement C5 ELISA Kit (Colorimetric) [NBP2-70049] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Mouse Complement C5 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse C5 in samples. No significant cross-reactivity or interference between Mouse C5 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.73%
Intra-Assay
CV% < 4.77%
Spike Recovery
84-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Complement C5 ELISA Kit (Colorimetric)

  • anaphylatoxin C5a analog
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
  • C5
  • complement C5
  • complement component 5
  • CPAMD4MGC142298
  • FLJ17816
  • FLJ17822

Background

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Mouse Complement C5 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol C5