![Western Blot: Collagen XXV alpha 1 Antibody [NB300-289] - Stable HEK293 cell line expressing CLAC-P using NB300-289. Lane 1: full-length CLAC-P (lysate). Lane 2: secreted form of CLAC-P (media). Lane 3: COLXIII (lysate). Lane 4 degrees COLXIII (media). Lane 5: COLXXIII (lysate). Lane 6: COLXXIII (media).](http://images.novusbio.com/fullsize/Collagen-XXV-alpha-1-Antibody-Western-Blot-NB300-289-img0003.jpg "Western Blot: Collagen XXV alpha 1 Antibody [NB300-289] - Stable HEK293 cell line expressing CLAC-P using NB300-289. Lane 1: full-length CLAC-P (lysate). Lane 2: secreted form of CLAC-P (media). Lane 3: COLXIII (lysate). Lane 4 degrees COLXIII (media). Lane 5: COLXXIII (lysate). Lane 6: COLXXIII (media).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide mapping to residues 430-443 [DYNGNLHEALQRIT] of the NC3 region of human and mouse CLAC-P. |
| Specificity | This detects full-length CLAC-P and the secreted form of CLAC-P protein. It does not react with full-length or secreted forms of COLXIII protein or the COLXXIII protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | COL25A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody has been tested in Western blot and immunohistochemistry-Paraffin. Suggested Starting Dilutions:* Western Blot: 0.2 ug/ml (ECL Overexpressed protein) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Collagen XXV alpha 1 Antibody (NB300-289)Find related products by research area.
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![Immunohistochemistry: Collagen XXV Antibody [NB300-289] - Immunostain of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) using NB 300-289. At high magnification, NB 300-289 stains senile plaques.](http://images.novusbio.com/fullsize/Collagen-XXV-Antibody-Immunohistochemistry-NB300-289-img0001.jpg "Immunohistochemistry: Collagen XXV Antibody [NB300-289] - Immunostain of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) using NB 300-289. At high magnification, NB 300-289 stains senile plaques.")
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