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Collagen XXV alpha 1 Antibody - BSA Free

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Western Blot: Collagen XXV Antibody [NB300-248] - Analysis of NC2-2 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100 kDa in SPs of Alzheimer diseased brain.
Western Blot: Collagen XXV Antibody [NB300-248] - Analysis of NC2-2 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100 kDa in SPs of Alzheimer diseased brain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB300-248AF350
Alexa Fluor 405
NB300-248AF405
Alexa Fluor 488
NB300-248AF488
Alexa Fluor 532
NB300-248AF532
Alexa Fluor 594
NB300-248AF594
Alexa Fluor 647
NB300-248AF647
Alexa Fluor 700
NB300-248AF700
Alexa Fluor 750
NB300-248AF750
Allophycocyanin
NB300-248APC
Biotin
NB300-248B
CoraFluor 1
NB300-248CL1
DyLight 350
NB300-248UV
DyLight 405
NB300-248V
DyLight 488
NB300-248G
DyLight 550
NB300-248R
DyLight 594
NB300-248DL594
DyLight 650
NB300-248C
DyLight 680
NB300-248FR
DyLight 755
NB300-248IR
FITC
NB300-248F
HRP
NB300-248H
Janelia Fluor 525
NB300-248JF525
Janelia Fluor 549
NB300-248JF549
Janelia Fluor 646
NB300-248JF646
Janelia Fluor 669
NB300-248JF669
PE
NB300-248PE
PerCP
NB300-248PCP
mFluor Violet 450 SE
NB300-248MFV450
mFluor Violet 500 SE
NB300-248MFV500
mFluor Violet 610 SE
NB300-248MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB300-248SS
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Collagen XXV alpha 1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide mapping to residues 155-169 [KGEQGDQGPRMVFPK] of the NC2-2 region of human and mouse CLAC-P.
Specificity
Reacts with residues 155-169 [KGEQGDQGPRMVFPK] of the NC2-2 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100 kDa in SPs of AD brain, but not in diffuse plaques of non-demented aged individuals, diffuse deposits in sub-cortical structures and cerebellum in AD nor in cerebral amyloid angiopathy
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL25A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Western blot - Use at 1:500 dilution. This antibody has only been tested in Western Blot. The investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Collagen XXV alpha 1 Overexpression Lysate

Reactivity Notes

Human and mouse. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Collagen XXV alpha 1 Antibody - BSA Free

  • Alzheimer disease amyloid-associated protein
  • AMY
  • CLAC
  • CLACP
  • CLAC-P
  • COL25A1
  • Collagen 25
  • collagen alpha-1(XXV) chain
  • Collagen XXV alpha 1
  • collagen, type XXV, alpha 1
  • Collagen-25
  • collagen-like Alzheimer amyloid plaque component
  • collagenous Alzheimer amyloid plaque component

Background

Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Collagen XXV alpha 1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL25A1
Entrez