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COBRA1 Antibody

Images

 
Western Blot: COBRA1 Antibody [H00025920-B01P] - Analysis of COBRA1 expression in human pancreas.
Immunocytochemistry/ Immunofluorescence: COBRA1 Antibody [H00025920-B01P] - Analysis of purified antibody to COBRA1 on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: COBRA1 Antibody [H00025920-B01P] - Analysis of purified antibody to COBRA1 on formalin-fixed paraffin-embedded human placenta. (antibody concentration 3 ug/ml)
Western Blot: COBRA1 Antibody [H00025920-B01P] - Analysis of COBRA1 expression in transfected 293T cell line by COBRA1 polyclonal antibody. Lane 1: COBRA1 transfected lysate(52.25 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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COBRA1 Antibody Summary

Immunogen
COBRA1 (AAH11892.1, 1 a.a. - 475 a.a.) full-length human protein. MPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL
Specificity
RP13-122B23.3 - cofactor of BRCA1,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
COBRA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. May also be used for immunofluorescence, immunohistochemistry (paraffin).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COBRA1 Antibody

  • cofactor of BRCA1DKFZp586B0519
  • KIAA1182negative elongation factor B
  • NELFB
  • NELF-Bnegative elongation factor protein B

Background

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COBRA1 Antibody (H00025920-B01P) (0)

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Secondary Antibodies

 

Isotype Controls

Additional COBRA1 Products

Array H00025920-B01P

Research Areas for COBRA1 Antibody (H00025920-B01P)

Find related products by research area.

Blogs on COBRA1.

COBRA1: A Key Player in Transcriptional Pausing
Co-factor of BRCA1, also known as COBRA1, was first identified as a protein that binds to the tumour suppressor protein encoded by the breast cancer susceptibility gene BRCA1 (1). It was subsequently found to be identical to subunit B of the Negative ...  Read full blog post.

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Bioinformatics

Gene Symbol COBRA1
Entrez
Uniprot