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Recombinant Human CNPase GST (N-Term) Protein

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SDS-Page: Recombinant Human CNPase Protein [H00001267-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, PAGE, AP

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Recombinant Human CNPase GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-421 of Human CNP

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
CNP
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
Theoretical MW
72.05 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using H00001267-P01.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CNPase GST (N-Term) Protein

  • 2', 3' cyclic nucleotide 3' phosphohydrolase
  • 2'-3'-cyclic nucleotide 3' phosphodiesterase
  • 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
  • CNP1
  • CNPase
  • EC 3.1.4.37

Background

CNP( AAH01362, 1 a.a. - 422 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for CNPase Recombinant Protein (H00001267-P01)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CNPase Recombinant Protein (H00001267-P01). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in the CNPase Recombinant Protein. However, we do not plan to use the protein for WB, ELISA or Protein Arrays, but we are interested in the enzymatic activity. Thus, I wanted to know, if this enzyme is still enzymatically active and cleaves 2',3'-cyclic nucleotides?
    • Unfortunately, H00001267-P01 is not guaranteed for use in enzyme assays. We distribute this product for a Taiwanese company called Abnova and their R&D team grows all of their proteins in a cell free wheat germ system. Because of this particular system, some of their proteins may not follow normal PTM or protein folding processing. We therefore generally do not recommend them for use in experiments that require protein activity. However, it may work in your particular experiment, but we would not be able to guarantee results.

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Bioinformatics

Gene Symbol CNP