Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.
CNPase Antibody [NB100-1935] - CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.
Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of CNPase shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences.
Localization
Oligodendrocyte
Marker
Oligodendrocyte Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
CNP
Purity
Immunogen affinity purified
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47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
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Purification Notes After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.
Storage Notes Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Alternate Names for CNPase Antibody
2', 3' cyclic nucleotide 3' phosphohydrolase
2'-3'-cyclic nucleotide 3' phosphodiesterase
2'-3'-cyclic-nucleotide 3'-phosphodiesterase
CNP1
CNPase
EC 3.1.4.37
Background
2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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