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CLPP Antibody

Images

 
Genetic Strategies: Western Blot: CLPP Antibody [NBP1-89557] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CLPP antibody. Remaining relative intensity is ...read more
Immunocytochemistry/ Immunofluorescence: CLPP Antibody [NBP1-89557] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Western Blot: CLPP Antibody [NBP1-89557] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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CLPP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MGSLLLAAGTPGMRHSLPNSRIMIHQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLPP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in IHC-Frozen was reported in scientific literature (PMID: 30877431).
Control Peptide
CLPP Recombinant Protein Antigen (NBP1-89557PEP)
Publications
Read Publications using
NBP1-89557 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22361696)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CLPP Antibody

  • ATP-dependent protease ClpAP, proteolytic subunit, human
  • ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog
  • ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli)
  • ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli)
  • ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog
  • EC 3.4.21.92
  • Endopeptidase Clp
  • putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

Background

CLPP belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CLPP Antibody (NBP1-89557)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CLPP Antibody (NBP1-89557) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CLPP Products

Research Areas for CLPP Antibody (NBP1-89557)

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Blogs on CLPP

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Bioinformatics

Gene Symbol CLPP