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Clathrin interactor 1 Antibody

Images

 
Independent Antibodies: Western Blot: Clathrin interactor 1 Antibody [NBP1-85570] - Analysis using Anti-CLINT1 antibody NBP1-85570 (A) shows similar pattern to independent antibody NBP2-49239 (B).
Western Blot: Clathrin interactor 1 Antibody [NBP1-85570] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Clathrin interactor 1 Antibody [NBP1-85570] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -Staining of human kidney shows strong cytoplasmic/ membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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Clathrin interactor 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DEEETVTTKHIHITQATETTTTRHKRTANPSKTIDLGAAAHYTGDKASPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADFGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLINT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Clathrin interactor 1 Protein (NBP1-85570PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Clathrin interactor 1 Antibody

  • clathrin interacting protein localized in the trans-Golgi region
  • clathrin interactor 1
  • Clathrin-interacting protein localized in the trans-Golgi region
  • CLINT
  • ENTHClint
  • enthoprotin
  • EPN4Enthoprotin
  • EPNRepsin 4
  • Epsin-4
  • EpsinR
  • Epsin-related protein
  • FLJ46753
  • KIAA0171epsinR

Background

Epsin R is enriched in clathrin-coated vesicles and associates with clathrin and AP1 (adaptor protein complex 1). EpsinR plays a role in transport of vesicles in the endosomal/trans-Golgi network and at the synapses of neurons. The EpsinR gene has been associated with the genetic susceptibility to schizophrenia. Alternative names for EpsinR include Epsin-4, Epsin-related protein, enthoprotin, clathrin interactor 1, Clint, CLINT1, ENTH, EPN4, EPNR, and KIAA0171.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CLINT1