![Immunoprecipitation: Citron Kinase Antibody [NBP1-28740] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Citron antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Citron was also immunoprecipitated by rabbit anti-Citron antibody BL5459, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Citron-Kinase-Antibody-Immunoprecipitation-NBP1-28740-img0001.jpg "Immunoprecipitation: Citron Kinase Antibody [NBP1-28740] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Citron antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Citron was also immunoprecipitated by rabbit anti-Citron antibody BL5459, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1925 and 1975 of human citron (rho-interacting, serine/threonine kinase 21) using the numbering given in entry NP_009105.1 (GeneID 11113). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CIT |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-28740 | Applications | Species |
|---|---|---|
| Hosono Y, Yamaguchi T, Mizutani E et al. MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis. The EMBO Journal. 2011-11-15 [PMID: 22085929] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Citron Kinase Antibody (NBP1-28740)Find related products by research area.
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