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Chitinase 3-like 1/YKL-40 Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF2649
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2649
Carrier Free
AF2649
Carrier Free
AF2649-SP
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Chitinase 3-like 1/YKL-40 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chitinase 3-like 1/YKL-40
Tyr22-Ala381
Accession # Q61362
Specificity
Detects mouse Chitinase 3-like 1/YKL-40 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human Chitinase 3‑like 1/YKL-40 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Chil1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publication using BAF2649.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by US Patent # 7,230,086 which is owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chitinase 3-like 1/YKL-40 Antibody [Biotin]

  • AW208766
  • Brp39
  • CHI3L1
  • Chitinase 3 like 1
  • Chitinase 3-like 1
  • gp39
  • HCgp39
  • YKL-40

Background

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
  16. Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Chitinase 3-like
1/YKL-40 Antibody [Bi ...
BAF2649
Chitinase 3-like 1/YKL-40 Antibody [Biotin]
Species: Mu
Applications: WB

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Bioinformatics

Gene Symbol Chil1
Uniprot