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cGAS Antibody

Images

 
Orthogonal Strategies: Western Blot: cGAS Antibody [NBP1-86761] - Analysis in human cell line CACO-2 and human cell line HEK 293
Simple Western: cGAS Antibody [NBP1-86761] - Simple Western lane view shows a specific band for MB21D1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Simple Western: cGAS Antibody [NBP1-86761] - Electropherogram image(s) of corresponding Simple Western lane view. MB21D1 antibody was used at 1:30 dilution on RT-4 lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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cGAS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQLKERF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MB21D1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Reactivity reported in scientific literature (PMID: 24970844]
  • Immunohistochemistry Reactivity reported in scientific literature (PMID: 35099823)
  • Simple Western 1:30
  • Western Blot 0.04 - 0.4 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
cGAS Recombinant Protein Antigen (NBP1-86761PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-86761 in the following applications:

Publications
Read Publications using
NBP1-86761 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for cGAS Antibody

  • C6orf150
  • c-GAS
  • cyclic GMP-AMP synthase
  • h-cGAS
  • Mab-21 domain containing 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cGAS Antibody (NBP1-86761)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-86761 Applications Species
Li K, Gong Y, Qiu D et al. Hyperbaric oxygen facilitates teniposide-induced cGAS-STING activation to enhance the antitumor efficacy of PD-1 antWBody in HCC Journal for immunotherapy of cancer 2022-08-01 [PMID: 36002188] (WB) WB
Lohinai Z, Dora D, Caldwell C et al. Loss of STING expression is prognostic in non-small cell lung cancer Journal of surgical oncology 2022-01-31 [PMID: 35099823] (IF/IHC, Human) IF/IHC Human
Deater M, Tamhankar M, Lloyd RE. TDRD3 is an antiviral restriction factor that promotes IFN signaling with G3BP1 PLOS Pathogens 2022-01-27 [PMID: 35085371]
Luzwick JW, Dombi E, Boisvert RA et al. MRE11-dependent instability in mitochondrial DNA fork protection activates a cGAS immune signaling pathway Science advances 2021-12-17 [PMID: 34910513]
Nicole Berndt, Christine Wolf, Kristina Fischer, Emanuel Cura Costa, Peter Knuschke, Nick Zimmermann, Franziska Schmidt, Martin Merkel, Osvaldo Chara, Min Ae Lee-Kirsch, Claudia Günther Photosensitivity and cGAS-Dependent IFN-1 Activation in Patients with Lupus and TREX1 Deficiency. The Journal of investigative dermatology 2022-05-09 [PMID: 34400195]
Schwertner B, Lindner G, Toledo Stauner C et al. Nectin-1 Expression Correlates with the Susceptibility of Malignant Melanoma to Oncolytic Herpes Simplex Virus In Vitro and In Vivo Cancers 2021-06-19 [PMID: 34205379] (IHC-P, Human) IHC-P Human
Chen H, Chen H, Zhang J et al. cGAS suppresses genomic instability as a decelerator of replication forks Sci Adv 2020-10-01 [PMID: 33055160] (ICC/IF, Human) ICC/IF Human
Verrier E R, Yim S A et al. Hepatitis B Virus Evasion From Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase Sensing in Human Hepatocytes. Hepatology 2018-01-11 [PMID: 29679386] (WB, Human) WB Human
Hansen K, Prabakaran T, Laustsen A et al. Listeria monocytogenes induces IFN-beta expression through an IFI16-, cGAS- and STING-dependent pathway. EMBO J 2014-08-01 [PMID: 24970844] (ICC/IF, Human) ICC/IF Human
Berg RK, Rahbek SH, Kofod-Olsen E et al. T Cells Detect Intracellular DNA but Fail to Induce Type I IFN Responses: Implications for Restriction of HIV Replication. PLoS One 2014-01-01 [PMID: 24404168]
Show All 11 Publications.

Review for cGAS Antibody (NBP1-86761) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-86761:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot cGAS NBP1-86761
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman primary hepatocytes
SpeciesHuman
LotG105753

Comments

CommentsName: Anti-cGAS antibody (NBP1-86761)
Catalog #: Anti-cGAS antibody (NBP1-86761)
Lot Number: Anti-cGAS antibody (NBP1-86761, Lot # G105753)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human primary hepatocytes; lane 2: Hep G2
Sample Information:
     Cell Line or Tissue: human primary hepatocytes, Hep G2
Species: human
     Treatment: No
Lysate Preparation:
     Date of lysate preparation: December 7, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 10%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/500
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Promega, cat # W401B, Lot # 0000187662
Secondary description: goat anti-rabbit secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot #n9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 3 min
Molecular weight of band(s): ~55 kDa
Experimental Concerns and Observations: Specific bands around 55 kDa were observed

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for cGAS Antibody (NBP1-86761) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
12/18/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol MB21D1