![Immunohistochemistry-Paraffin: CFTR Antibody (SPM176) [NBP2-53373] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (SPM176).](http://images.novusbio.com/fullsize/CFTR-Antibody-SPM176-Immunohistochemistry-Paraffin-NBP2-53373-img0001.jpg "Immunohistochemistry-Paraffin: CFTR Antibody (SPM176) [NBP2-53373] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CFTR Monoclonal Antibody (SPM176).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | SPM176 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54504) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human CFTR fragment (Uniprot: P13569) |
| Localization | Cell Surface and Cytoplasmic |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CFTR |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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