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CDX2 Antibody (CDX2/1690) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472] - Gastric Adenocarcinoma stained with CDX2 antibody. pH 9 HIER antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-54472] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).
Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-54472] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, MA, CyTOF-ready
Clone
CDX2/1690
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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CDX2 Antibody (CDX2/1690) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53247).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (around aa150-249) of human CDX2 protein (exact sequence is proprietary) (Uniprot: Q99626)
Localization
Nuclear
Marker
GI Epithelial Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDX2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 2 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Protein Array
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-54472 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CDX2 Antibody (CDX2/1690) - Azide and BSA Free

  • caudal type homeo box transcription factor 2
  • caudal type homeobox 2
  • Caudal-type homeobox protein 2
  • CDX2
  • CDX3
  • CDX-3
  • CDX3caudal type homeobox transcription factor 2
  • homeobox protein CDX-2

Background

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDX2 Antibody (NBP2-54472) (0)

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Review for CDX2 Antibody (NBP2-54472) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-54472:
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Immunofluorescence - paraffin
(1)
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Human
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Images Ratings Applications Species Date Details
Other CDX2 NBP2-54472
Enlarge
3
reviewed by:
Verified Customer
Immunofluorescence - paraffin Human 01/20/2019
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Summary

ApplicationOther
Sample Testedhuman gastric tumor section
SpeciesHuman
LotI don't have it any more

Comments

CommentspH 9 antigen retrieval

Product General Protocols

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FAQs for CDX2 Antibody (NBP2-54472) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
01/20/2019
Application: Immunofluorescence - paraffin
Species: Human

Bioinformatics

Gene Symbol CDX2