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CD46 Antibody (122.2) [PE/Cy5.5]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, CyTOF-ready
Clone
122.2
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy5.5

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-33159AF350
Alexa Fluor 405
NBP2-33159AF405
Alexa Fluor 488
NBP2-33159AF488
Alexa Fluor 532
NBP2-33159AF532
Alexa Fluor 594
NBP2-33159AF594
Alexa Fluor 647
NBP2-33159AF647
Alexa Fluor 700
NBP2-33159AF700
Alexa Fluor 750
NBP2-33159AF750
Allophycocyanin
NBP2-33159APC
Allophycocyanin/Cy7
NBP2-33159APCCY7
Biotin
NBP2-33159B
DyLight 350
NBP2-33159UV
DyLight 405
NBP2-33159V
DyLight 488
NBP2-33159G
DyLight 550
NBP2-33159R
DyLight 594
NBP2-33159DL594
DyLight 650
NBP2-33159C
DyLight 680
NBP2-33159FR
DyLight 755
NBP2-33159IR
FITC
NBP2-33159F
Janelia Fluor 549
NBP2-33159JF549
Janelia Fluor 646
NBP2-33159JF646
PE
NBP2-33159PE
PE/Atto594
NBP2-33159PEATT594
PE/Cy7
NBP2-33159PECY7
PerCP
NBP2-33159PCP
Unconjugated
NBP2-25222
mFluor Violet 450 SE
NBP2-33159MFV450
mFluor Violet 500 SE
NBP2-33159MFV500
mFluor Violet 610 SE
NBP2-33159MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-25222
Azide and BSA Free
NBP2-33159
IHC-Prediluted
NBP2-44670
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CD46 Antibody (122.2) [PE/Cy5.5] Summary

Additional Information
Clone 122.2 was used by HLDA to establish CD designation.
Immunogen
Recombinant human CD46 protein (Uniprot: P15529)
Localization
Cell surface
Marker
T-Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD46
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD46 Antibody (122.2) [PE/Cy5.5]

  • AHUS2
  • CD46 antigen
  • CD46 molecule, complement regulatory protein
  • CD46
  • MCP
  • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
  • MIC10
  • TLXcomplement regulatory protein
  • trophoblast leucocyte common antigen
  • Trophoblast leukocyte common antigen
  • trophoblast-lymphocyte cross-reactive antigen

Background

CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in regulation of complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD46