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Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

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ELISA: CD40 Ligand/TNFSF5 ELISA Kit [NBP1-91184] - Standard curve
ELISA: CD40 Ligand/TNFSF5 ELISA Kit [NBP1-91184] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.06 ng/mL

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Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) Summary

Description
The human sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40L
Specificity
The assay detects both natural and recombinant human CD40 Ligand/TNFSF5. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD40 Ligand/TNFSF5 positive sample. There was no cross reactivity or interference.
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.06 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 6.80%
Intra-Assay
CV% < 4.00%
Spike Recovery
91%
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CD40LG

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40L
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Blue-Dye
  5. Control high, lyophilized
  6. Control low, lyophilized
  7. Dilution Plates
  8. Green-Dye
  9. HRP-Conjugate anti-human sCD40L monoclonal antibody
  10. Human sCD40L Standard lyophilized, 20 ng/ml upon reconstitution
  11. Sample Diluent
  12. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.) A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40. CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for CD40 Ligand/TNFSF5 ELISA Kit (NBP1-91184). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
    • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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  Manual - Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol CD40LG