![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 1283D |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | This CD36 Antibody (1283D) was developed against a synthetic peptide made to an internal portion of the human CD36 protein (between amino acids 100-150) [Uniprot P16671] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CD36 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 110 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP2-54790 | Applications | Species |
|---|---|---|
| Hao JW, Wang J, Guo H et al. CD36 facilitates fatty acid uptake by dynamic palmitoylation-regulated endocytosis Nat Commun 2020-09-21 [PMID: 32958780] (IP) | IP |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD36 Antibody (NBP2-54790)Find related products by research area.
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The affects of Perilipin 2 on diet and metabolism Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo... Read full blog post. |
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Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1) SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage... Read full blog post. |
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How do Lipase A and the CD36 Antibody Relate to Each Other Obesity, diabetes and metabolic disorders are dramatically on the increase, linked to disorders such as heart disease, stroke and cancer. To combat this, research groups are studying metabolism at both a cellular and a systemic level. Although we at N... Read full blog post. |
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ABCA1 Mutations: A Risk Factor in Atherosclerosis Related Strokes The ATP Binding Cassette Transporter (ABCA1) gene encodes the cholesterol regulatory efflux protein, which plays a key role in lipid metabolism. ABCA1 antibody products are an important part of any antibody catalog covering atherosclerosis disease res... Read full blog post. |
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| Gene Symbol | CD36 |
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg "Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.")
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - 3T3-L1 adipocytes were pretreated and treated with ML348 (10 uM) for 1 h, followed by BSA or oleate treatment for another 1 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD36-Antibody-1283D-BSA-Free-Western-Blot-NBP2-54790-img0024.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - 3T3-L1 adipocytes were pretreated and treated with ML348 (10 uM) for 1 h, followed by BSA or oleate treatment for another 1 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780/) licensed under a CC-BY license.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg "Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A Thrombospondin-1 [HRP]](https://images.novusbio.com/images2/Thrombospondin-1_DTSP10_ELISA_199.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images2/ICAM1_AF796_Immunohistochemistry_17354.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-4120241048112.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-41202410481112.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Immunocytochemistry FATP1/SLC27A1 Antibody (308420) [Unconjugated]](https://images.novusbio.com/images2/FATP1_MAB3304_Immunocytochemistry__Immunofluorescence_19621.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/CD14_DC140_ELISA_42.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/DATA_CD14_DC140_ELISA_597.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")