I potentially am interested in CD31/PECAM1 Antibody (TLD-3A12). I am woundering if this AB is suitable to use for immunohistochemistry with PFA-fixed section. Is it suitable?

Name: CD31/PECAM-1 Antibody (TLD-3A12)
Brand: Novus Biologicals
SKU: NB100-64796
Rating: 5 (1 reviews)

Question

Accepted Answer

Yes, this antibody is suitable for IHC with PFA

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	RtSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC
Clone	TLD-3A12
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-64796AF350

Alexa Fluor 405 NB100-64796AF405

Alexa Fluor 488 NB100-64796AF488

Alexa Fluor 532 NB100-64796AF532

Alexa Fluor 594 NB100-64796AF594

Alexa Fluor 647 NB100-64796AF647

Alexa Fluor 700 NB100-64796AF700

Alexa Fluor 750 NB100-64796AF750

Allophycocyanin NB100-64796APC

Biotin NB100-64796B

Biotin NB100-63701

DyLight 350 NB100-64796UV

DyLight 405 NB100-64796V

DyLight 488 NB100-64796G

DyLight 550 NB100-64796R

DyLight 594 NB100-64796DL594

DyLight 650 NB100-64796C

DyLight 680 NB100-64796FR

DyLight 755 NB100-64796IR

FITC NB100-63703-100ug

FITC NB100-64796F

HRP NB100-64796H

Janelia Fluor 525 NB100-64796JF525

Janelia Fluor 549 NB100-64796JF549

Janelia Fluor 646 NB100-64796JF646

Janelia Fluor 669 NB100-64796JF669

PE NB100-64796PE

PerCP NB100-64796PCP

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64796AF350
Alexa Fluor 405	NB100-64796AF405
Alexa Fluor 488	NB100-64796AF488
Alexa Fluor 532	NB100-64796AF532
Alexa Fluor 594	NB100-64796AF594
Alexa Fluor 647	NB100-64796AF647
Alexa Fluor 700	NB100-64796AF700
Alexa Fluor 750	NB100-64796AF750
Allophycocyanin	NB100-64796APC
Biotin	NB100-64796B
Biotin	NB100-63701
DyLight 350	NB100-64796UV
DyLight 405	NB100-64796V
DyLight 488	NB100-64796G
DyLight 550	NB100-64796R
DyLight 594	NB100-64796DL594
DyLight 650	NB100-64796C
DyLight 680	NB100-64796FR
DyLight 755	NB100-64796IR
FITC	NB100-63703-100ug
FITC	NB100-64796F
HRP	NB100-64796H
Janelia Fluor 525	NB100-64796JF525
Janelia Fluor 549	NB100-64796JF549
Janelia Fluor 646	NB100-64796JF646
Janelia Fluor 669	NB100-64796JF669
PE	NB100-64796PE
PerCP	NB100-64796PCP

Immunogen	Activated, Lewis rat derived microglial cells
Localization	Membrane; single pass type I membrane protein.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	PECAM1
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

CD31/PECAM-1 Antibody (TLD-3A12)

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Publications for CD31/PECAM-1 Antibody (NB100-64796)(60)

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Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.09% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein A purified

Publications using NB100-64796	Applications	Species
Zhan X, Wen Z, Chen Y et al. Protocol to assemble metal-phenolic framework nanoparticles based on polyphenol-mediated biomimetic mineralization for wound healing in rats STAR protocols 2023-02-24 [PMID: 36861839] (IHC, Mouse)	IHC	Mouse
Spyridakos D, Mastrodimou N, Vemuri K et al. Blockade of CB1 or Activation of CB2 Cannabinoid Receptors Is Differentially Efficacious in the Treatment of the Early Pathological Events in Streptozotocin-Induced Diabetic Rats International Journal of Molecular Sciences 2022-12-23 [PMID: 36613692] (IHC-Fr, Rat)	IHC-Fr	Rat
Lu W, Zhao R, Fan X et al. Time-varying characteristics of the induced membrane and its effects on bone defect repair Injury 2022-12-24 [PMID: 36581479]
Wang H, Li X, Lai S et al. Construction of Vascularized Tissue Engineered Bone with nHA-Coated BCP Bioceramics Loaded with Peripheral Blood-Derived MSC and EPC to Repair Large Segmental Femoral Bone Defect ACS applied materials & interfaces 2022-12-22 [PMID: 36548196]
Mani AM, Dhanabalan K, Lamin V et al. BAG3 Attenuates Ischemia-Induced Skeletal Muscle Necroptosis in Diabetic Experimental Peripheral Artery Disease International Journal of Molecular Sciences 2022-09-14 [PMID: 36142618] (Immunohistochemistry)	Immunohistochemistry
Zhang L, Tai Y, Liu X Et al. Natural polymeric and peptide-loaded composite wound dressings for scar prevention Applied Materials Today 2021-12-01
Zhou m, Li Y, Tang S Dual orexin receptor antagonists and Tgn-020 affect learning and memory in 8-mth-old APP / PS1 (AD) mice Research Square 2022-12-09 (IHC, Mouse)	IHC	Mouse
Celikten M, Sahin H, Senturk GE et al. The Effect of Platelet-Rich Fibrin, Platelet-Rich Plasma, and Concentrated Growth Factor in the Repair of Full Thickness Rotator Cuff Tears Journal of shoulder and elbow surgery 2023-10-26 [PMID: 37898418] (IHC-P, Rabbit)	IHC-P	Rabbit
Jin H, Yang B, Jiang D et al. Inhibitory effect of anti-Scg3 on corneal neovascularization: a preliminary study BMC Ophthalmology 2022-11-28 [PMID: 36443679] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Lamin V, Verry J, Dokun OS et al. microRNA-29a Regulates ADAM12 Through Direct Interaction With ADAM12 mRNA and Modulates Postischemic Perfusion Recovery Journal of the American Heart Association 2022-08-16 [PMID: 35946473] (IHC-P, Mouse) Details: IHC-P 1:1000 dilution	IHC-P	Mouse
Tamura S, Mantani Y, Nakanishi S et al. Region specificity of fibroblast-like cells in the mucosa of the rat large intestine Cell and tissue research 2022-07-02 [PMID: 35779135]
Xu L, Lu X, Xiao P et al. Interleukin-17A Contributed to the Damage of Blood-CNS Barriers During Streptococcus suis Meningitis Molecular neurobiology 2022-01-19 [PMID: 35044625]
Lamin V, Verry J, Eigner-Bybee I et al. Modulation of miR-29a and ADAM12 Reduces Post-Ischemic Skeletal Muscle Injury and Improves Perfusion Recovery and Skeletal Muscle Function in a Mouse Model of Type 2 Diabetes and Peripheral Artery Disease International journal of molecular sciences 2021-12-31 [PMID: 35008854] (IHC-P, Mouse)	IHC-P	Mouse
Wu C, Du M, Yu R et al. A novel mechanism linking ferroptosis and endoplasmic reticulum stress via the circPtpn14/miR-351-5p/5-LOX signaling in melatonin-mediated treatment of traumatic brain injury Free radical biology & medicine 2022-01-01 [PMID: 34883251] (ICC/IF, Mouse, Human)	ICC/IF	Mouse, Human
Tigges J, Eggerbauer F, Worek F Et al. Optimization of long-term cold storage of rat precision cut lung slices (PCLS) with a tissue preservation solution American journal of physiology. Lung cellular and molecular physiology 2021-10-13 [PMID: 34643087] (FLOW, Rat)	FLOW	Rat
Cheng YQ, Wu CR, Du MR et al. CircLphn3 protects the blood-brain barrier in traumatic brain injury Neural regeneration research 2022-04-01 [PMID: 34472480] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen YH, Rao ZF, Liu YJ et al. Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing Biomolecules 2021-05-08 [PMID: 34066859] (IHC-Fr, Rat)	IHC-Fr	Rat
Parfenov AI, Krums LM, Bykova SV, Ahmadullina OV. [Chronic intestinal pseudo-obstruction]. Terapevticheskii arkhiv 2021-03-17 [PMID: 33720566]
Zhao B, Liu X, Ding H et al. VEGF-PLGA controlled-release microspheres enhanced angiogenesis in encephalomyosynangiosis-based chronic cerebral hypoperfusion Journal of Clinical Neuroscience 2020-11-01 [PMID: 33222901] (IHC-Fr, Rat)	IHC-Fr	Rat
Jiang F, Zhang W, Zhou M et al. Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration Theranostics 2020-06-03 [PMID: 32483449] (ICC/IF, Rat)	ICC/IF	Rat
Yue C, Guo Z et al. c-Jun Overexpression Accelerates Wound Healing in Diabetic Rats by Human Umbilical Cord-Derived Mesenchymal Stem Cells. Stem Cells Int 2020-05-14 [PMID: 32399050] (IF/IHC, Rat)	IF/IHC	Rat
Boscolo Sesillo F, Wong M, Cortez A, Alperin M. Isolation of muscle stem cells from rat skeletal muscles Stem Cell Research 2020-03-01 [PMID: 31931473]
Langendorf EK, Klein A, Drees P Exposure to radial extracorporeal shockwaves induces muscle regeneration after muscle injury in a surgical rat model J. Orthop. Res. 2019-12-16 [PMID: 31840830] (IF/IHC, Rat)	IF/IHC	Rat
Qayoom A, Aneesha VA, Anagha S et al. Lecithin-based deferoxamine nanoparticles accelerated cutaneous wound healing in diabetic rats Eur. J. Pharmacol. 2019-06-20 [PMID: 31228457] (IF/IHC, Rat)	IF/IHC	Rat
Wang L, Wang F, Zhao L et al. Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats Stem Cells Int. 2019-01-27 [PMID: 30833970] (IF/IHC, Rat)	IF/IHC	Rat
Chen B, Zhang J, Nie D et al. Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells. J. Cell. Biochem. 2018-11-01 [PMID: 30387227] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Matsuyoshi Y, Akahoshi M, Nakamura M et al. Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation Methods Mol. Biol. 2019-10-28 [PMID: 30367410] (Rat)		Rat
Jiang K, Chaimov D, Patel SN et al. 3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture. Biomaterials 2018-09-07 [PMID: 30224090] (ICC/IF)	ICC/IF
Sung JS, Corben AD, Brooks JD et al. Histopathologic characteristics of background parenchymal enhancement (BPE) on breast MRI Breast Cancer Res. Treat. 2018-08-23 [PMID: 30140962] (IHC-P, Human)	IHC-P	Human
Nadolski GJ, Hunt SJ, Weber CN et al. Near-Infrared Fluorescence Imaging of Matrix Metalloproteinase 2 Activity as a Biomarker of Vascular Remodeling in Hemodialysis Access. J Vasc Interv Radiol. 2018-07-27 [PMID: 30061060] (IHC-P, Rat)	IHC-P	Rat
Xing S, Pan N, Xu W et al. EphrinB2 activation enhances angiogenesis, reduces amyloid-b deposits and secondary damage in thalamus at the early stage after cortical infarction in hypertensive rats. J. Cereb. Blood Flow Metab. 2018-01-01 [PMID: 29624118] (Rat)		Rat
Mantani Y, Nishida M, Yamamoto K et al. Ultrastructural and Immunohistochemical Study on the Lamina Propria Cells Beneath Paneth Cells in the Rat Ileum. Anat Rec (Hoboken) 2018-06-01 [PMID: 29316348]
Eltony SA, Abdelhameed SY Effect of chronic administration of sildenafil citrate (Viagra) on the histology of the retina and optic nerve of adult male rat Tissue Cell 2017-02-27 [PMID: 28237322] (IHC-P, Human)	IHC-P	Human
Pacheco-Quinto J, Eckman CB, Eckman EA. Major amyloid-beta-degrading enzymes, endothelin-converting enzyme-2 and neprilysin, are expressed by distinct populations of GABAergic interneurons in hippocampus and neocortex. Neurobiol Aging 2016-08-20 [PMID: 27644077]
Robbins AK, Mateson AB, Khandha A et al. Fetal Rat Gubernaculum Mesenchymal Cells Adopt Myogenic and Myofibroblast-Like Phenotypes. J Urol 2016-07-01 [PMID: 26748163]
Ram M, Singh V, Kumawat S et al. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int. Immunopharmacol. 2016-01-01 [PMID: 26679676] (IHC-P, Rat) Details: VEGF antibody (clone VG1) was used at 1.5 ug/ml concentration for the analysis of granulation/healing tissues of control and bilirubin-treated diabetic rats (Fig. 4).	IHC-P	Rat
Ram M, Singh V, Kumawat S et al. Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats. Eur J Pharmacol 2015-10-05 [PMID: 26101070] (Rat)		Rat
Kant V, Gopal A, Kumar D et al. Curcurmin-induced angiogenesis hastens wound healing in diabetic rats. J Surg Res. 2014-10-17 [PMID: 25454972] (IHC-P, Rat) Details: This citation used the Biotin form of this antibody.	IHC-P	Rat
Xue S, Wu G, Zhang H et al. Transplantation of adipocyte-derived stem cells in a hydrogel scaffold for the repair of cortical contusion injury in rats. J Neurotrauma. 2014-09-16 [PMID: 25225747]
Li Wenjuan, Zhao Ruxing, Liu Jidong et al. Small islets transplantation superiority to large ones: implications from islet microcirculation and revascularization. J Diabetes Res. 2014-04-16 [PMID: 24829922] (IF/IHC, Rat)	IF/IHC	Rat
Gopal A, Kant V, Gopalakrishnan A et al. Chitosan-based copper nanocomposite accelerates healing in excision wound model in rats. Eur. J. Pharmacol. 2014-03-13 [PMID: 24632085] (IHC-P, Rat)	IHC-P	Rat
Kant V, Gopal A, Kumar D et al. Topical pluronic F-127 gel application enhances cutaneous wound healing in rats. Acta Histochem 2013-05-23 [PMID: 23706531] (IHC-P, Rat)	IHC-P	Rat
Salehi-Had H, Roh MI, Giani A et al. Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization PLoS One 2011-04-01 [PMID: 21559527] Details: This citation used the FITC version of this antibody.
Nakao A, Huang CS, Stolz DB et al. Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts. Cardiovasc Res. 2011-02-01 [PMID: 20851811]
Willis CL, Meske DS, Davis TP et al. Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation. J Cereb Blood Flow Metab. 2010-11-01 [PMID: 20700133]
Lochhead JJ, McCaffrey G, Quigley CE et al. Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation. J Cereb Blood Flow Metab. 2010-09-01 [PMID: 20234382]
Thebault P, Lhermite N, Tilly G et al. The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation. J Immunol. 2009-09-01 [PMID: 19667084]
Stevenson KS, McGlynn L, Hodge M et al. Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 2009-12-01 [PMID: 19326970]
Schilte, MN et al. Long-term intervention with heparins in a rat model of peritoneal dialysis. Perit Dial Int 29: 26-35. 2009-01-01 [PMID: 19164250]
Ohnishi, T et al. Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans. Toxicol Pathol 35: 904-9. 2007-01-01 [PMID: 18098037]
Fujimoto, KL et al. An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction. J Am Coll Cardiol 49: 2292-300. 2007-01-01 [PMID: 17560295]
Wilson, E et al. An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J Immunol Methods 322: 137-42. 2007-01-01 [PMID: 17362980] (IHC-P)	IHC-P
Ott, I et al. Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction. FASEB J 19: 992-4. 2005-01-01 [PMID: 15814609]
Nakao, A et al. Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury. Am J Pathol 163: 1587-98. 2003-01-01 [PMID: 14507665]
Haywood, L et al. Inflammation and angiogenesis in osteoarthritis. Arthritis Rheum 48: 2173-7. 2003-01-01 [PMID: 12905470]
Seegers, HC et al. Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation. J Pharmacol Exp Ther 306: 8-12. 2003-01-01 [PMID: 12660314]
Kielian T, Hickey WF. Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development. Am J Pathol. 2000-08-01 [PMID: 10934167]
Graham, MJ et al. In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration. J Pharmacol Exp Ther 286: 447-58. 1998-01-01 [PMID: 9655890]
Williams, KC et al. PECAM-1 CD31 expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J Neurosci Res 456:747-57. 1996-01-01 [PMID: 8892086]
Flaris, NA et al. Characterisation of microglia and macrophages in the central nervous system of the rat. GLIA 7: 34-40. 1993-01-01 [PMID: 3471710]
Show All 60 Publications. Collapse Publications.

	Vinay Kant 05/29/2015
Application:	ICC
Species:	Rat

Gene Symbol	PECAM1
Uniprot	Q3SWT0(Rat)