CD31/PECAM-1 Antibody (MEC 7.46)

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, In Vivo.

Showing Publications 1 - 10 of 29. Show All 29 Publications. Collapse Publications.

Publications using NB100-1642	Applications	Species
Gau D, Daoud A, Allen A et al. Vascular endothelial profilin-1 drives a pro-tumorigenic tumor microenvironment and tumor progression in renal cancer The Journal of biological chemistry 2023-07-12 [PMID: 37451478] (IHC, Mouse)	IHC	Mouse
Acharya BR, Fang JS, Jeffery ED et al. Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest Life science alliance 2023-08-01 [PMID: 37197981]
O'Brien A, Zhou T, White T et al. FGF1 Signaling Modulates Biliary Injury and Liver Fibrosis in the Mdr2-/- Mouse Model of Primary Sclerosing Cholangitis Hepatology communications 2022-03-10 [PMID: 35271760]
Meng R, Cai WK, Xu WM et al. Generation and identification of endothelial-specific Hrh2 knockout mice Transgenic research 2021-03-30 [PMID: 33786748] (IF/IHC, Mouse)	IF/IHC	Mouse
Bhatti FUR, Dadwal UC, Valuch CR et al The effects of high fat diet, bone healing, and BMP-2 treatment on endothelial cell growth and function Bone 2021-02-13 [PMID: 33581374] (ICC/IF, Mouse) Details: Citation using the DyLight 488 version of this antibody.	ICC/IF	Mouse
Li Calzi S, Shaw LC, Moldovan L et al. Progenitor cell combination normalizes retinal vascular development in the oxygen-induced retinopathy (OIR) model JCI Insight 2019-11-02 [PMID: 31672944] (ICC/IF, Mouse)	ICC/IF	Mouse
Serra A, Gallart-Palau X, Park J et al. Vascular Bed Molecular Profiling by Differential Systemic Decellularization In Vivo. Arterioscler Thromb Vasc Biol 2018-01-10 [PMID: 30354219] (ICC/IF, Mouse)	ICC/IF	Mouse
Liu X, Hu J, Li Y et al. Mesenchymal stem cells expressing interleukin-18 inhibit breast cancer in a mouse model. Oncol Lett 2018-05-01 [PMID: 29725393]
Liang H, Deng L, Hou Y et al. Host STING-dependent MDSC mobilization drives extrinsic radiation resistance Nat Commun. 2017-11-23 [PMID: 29170400] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Katori S, Noguchi-Katori Y, Itohara S, Iwasato T. Spinal RacGAP alpha-Chimaerin Is Required to Establish the Midline Barrier for Proper Corticospinal Axon Guidance J. Neurosci. 2017-08-09 [PMID: 28747385] (IF/IHC, Mouse)	IF/IHC	Mouse
Ielacqua GD, Schlegel F, Fuchtemeier M et al. Magnetic Resonance Q Mapping Reveals a Decrease in Microvessel Density in the arcAbeta Mouse Model of Cerebral Amyloidosis. Front Aging Neurosci 2015-01-01 [PMID: 26834622] (IF/IHC, Mouse)	IF/IHC	Mouse
Yang P, Hong MS, Fu C et al. Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions. Surgery 2016-02-01 [PMID: 26387788]
Haxho F, Allison S, Alghamdi F et al. Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth, and metastasis in mouse model of human triple-negative breast adenocarcinoma. Breast Cancer Dove Med Press 2015-12-19 [PMID: 25525387] (IHC-P, Mouse)	IHC-P	Mouse
Burgi S, Seuwen A, Keist R et al. In Vivo Imaging of Hypoxia-Inducible Factor Regulation in a Subcutaneous and Orthotopic GL261 Glioma Tumor Model Using a Reporter Gene Assay. Mol Imaging. 2014-09-01 [PMID: 25248521]
Kalet BT, Anglin SR, Handschy A et al. Transcription Factor Ets1 Cooperates with Estrogen Receptor alpha to Stimulate Estradiol-Dependent Growth in Breast Cancer Cells and Tumors. PLoS One 2013-07-09 [PMID: 23874775] (ICC/IF, Mouse)	ICC/IF	Mouse
Lampugnani MG, Zanetti A, Breviario F, Balconi G, Orsenigo F, Corada M, Spagnuolo R, Betson M, Braga V, Dejana E. VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam. Mol Biol Cell;13(4):1175-89. 2002-04-01 [PMID: 11950930] (ICC/IF, Mouse)	ICC/IF	Mouse
Zips D, Eicheler W, Bruchner K, Jackisch T, Geyer P, Petersen C, van der Kogel AJ, Baumann M. Impact of the tumour bed effect on microenvironment, radiobiological hypoxia and the outcome of fractionated radiotherapy of human FaDu squamous-cell carcinoma growing in the nude mouse. Int J Radiat Biol;77(12):1185-93. 2001-12-01 [PMID: 11747543]
Leenders W, van Altena M, Lubsen N, Ruiter D, De Waal R. In vivo activities of mutants of vascular endothelial growth factor (VEGF) with differential in vitro activities. Int J Cancer;91(3):327-33. 2001-02-01 [PMID: 11169955] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Balconi G, Spagnuolo R, Dejana E. Development of endothelial cell lines from embryonic stem cells: A tool for studying genetically manipulated endothelial cells in vitro. Arterioscler Thromb Vasc Biol;20(6):1443-51. 2000-06-01 [PMID: 10845856] (FLOW, Mouse)	FLOW	Mouse
Del Maschio A, De Luigi A, Martin-Padura I, Brockhaus M, Bartfai T, Fruscella P, Adorini L, Martino G, Furlan R, De Simoni MG, Dejana E. Leukocyte recruitment in the cerebrospinal fluid of mice with experimental meningitis is inhibited by an antibody to junctional adhesion molecule (JAM). J Exp Med;190(9):1351-6. 1999-11-01 [PMID: 10544206] (In Vivo, Mouse)	In Vivo	Mouse
Corada M, Mariotti M, Thurston G, Smith K, Kunkel R, Brockhaus M, Lampugnani MG, Martin-Padura I, Stoppacciaro A, Ruco L, McDonald DM, Ward PA, Dejana E. Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo. Proc Natl Acad Sci U S A;96(17):9815-20. 1999-08-17 [PMID: 10449777] (ICC/IF, In Vivo, Mouse)	ICC/IF, In Vivo	Mouse
Bussink J, Kaanders JH, Rijken PF, Peters JP, Hodgkiss RJ, Marres HA, van der Kogel AJ. Vascular architecture and microenvironmental parameters in human squamous cell carcinoma xenografts: effects of carbogen and nicotinamide. Radiother Oncol;50(2):173-84. 1999-02-01 [PMID: 10368041]
Westphal JR, van't Hullenaar RG, van der Laak JA, Cornelissen IM, Schalkwijk LJ, van Muijen GN, Wesseling P, de Wilde PC, Ruiter DJ, de Waal RM. Vascular density in melanoma xenografts correlates with vascular permeability factor expression but not with metastatic potential. Br J Cancer;76(5):561-70. 1997-01-01 [PMID: 9303353] (IF/IHC, Mouse)	IF/IHC	Mouse
Dong QG, Bernasconi S, Lostaglio S et al. A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants. Arterioscler Thromb Vasc Biol. 1997-08-01 [PMID: 9301641] (FLOW, Mouse)	FLOW	Mouse
Floss T, Arnold HH, Braun T. A role for FGF-6 in skeletal muscle regeneration. Genes Dev;11(16):2040-51. 1997-08-15 [PMID: 9284044] (IF/IHC, Mouse)	IF/IHC	Mouse
Aizawa E Characterization of in vitro systems to generate oocytes and substitutes of sperm from pluripotent stem cells Thesis 2019-01-01
Vecchi A, Garlanda C, Lampugnani MG, Resnati M, Matteucci C, Stoppacciaro A, Schnurch H, Risau W, Ruco L, Mantovani A et al. Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Eur J Cell Biol;63(2):247-54. 1994-04-01 [PMID: 8082649] (Mouse)		Mouse
Tallman MM Adult and pediatric brain tumors targeted with the small molecule drug CBL0137 Thesis 2023-01-01 (IHC)	IHC
Potgens AJ, van Altena MC, Lubsen NH, Ruiter DJ, de Waal RM. Analysis of the tumor vasculature and metastatic behavior of xenografts of human melanoma cell lines transfected with vascular permeability factor. Am J Pathol;148(4):1203-17. 1996-04-01 [PMID: 8644861] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Show All 29 Publications. Collapse Publications.

Array NB100-1642

• CD31/PECAM-1 Antibodies
• CD31/PECAM-1 Antibody Pair
• CD31/PECAM-1 ELISA Kits
• CD31/PECAM-1 Lysate
• CD31/PECAM-1 Protein
• CD31/PECAM-1 Proteins and Enzymes
• CD31/PECAM-1 ELISAs