CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) - Azide and BSA Free

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC-P.

Showing Publications 1 - 7 of 7.

Publications using NBP2-47785	Applications	Species
Mascharak S, Guo JL, Foster DS et al. Desmoplastic stromal signatures predict patient outcomes in pancreatic ductal adenocarcinoma Cell reports. Medicine 2023-10-11 [PMID: 37865092]
Wabel E, Orr A, Flood ED et al. Chemerin is resident to vascular tunicas and contributes to vascular tone American journal of physiology. Heart and circulatory physiology 2023-07-01 [PMID: 37294893]
D Phillips, M Matusiak, BR Gutierrez, SS Bhate, GL Barlow, S Jiang, J Demeter, KS Smythe, RH Pierce, SP Fling, N Ramchurren, MA Cheever, Y Goltsev, RB West, MS Khodadoust, YH Kim, CM Schürch, GP Nolan Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma Nature Communications, 2021-11-18;12(1):6726. 2021-11-18 [PMID: 34795254]
Wu CT, Lidsky PV, Xiao Y et al. SARS-CoV-2 infects human pancreatic beta cells and elicits beta cell impairment Cell Metab 2021-06-03 [PMID: 34081912] (ICC/IF, Human) Details: Citation using the Azide and BSA Free format of this antibody.	ICC/IF	Human
Fan F, Malvestiti S, Vallet S et al. JunB is a key regulator of multiple myeloma bone marrow angiogenesis Leukemia 2021-05-19 [PMID: 34007044] Details: Citation using the Azide and BSA Free format of this antibody.
Lee IT, Nakayama T, Wu CT et al ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs Nat Commun 2020-10-29 [PMID: 33116139] (IHC-P, IHC-P, Human) Details: Citation using the Azide and BSA Free version of this antibody.	IHC-P, IHC-P	Human
Lee IT, Nakayama T, Wu CT et al. Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers medRxiv 2020-01-01 [PMID: 32511516] (IHC-P, Human) Details: Citation using the Azide and BSA Free format of this antibody.	IHC-P	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	Immunofluorescence - paraffin	Human	11/18/2018	View Summary Application Other Sample Tested Liver cancer tissue Species Human Lot I don't have it any more Comments Comments pH 9 antigen retrieval

Array NBP2-47785

• CD31/PECAM-1 Antibodies
• CD31/PECAM-1 Antibody Pair
• CD31/PECAM-1 ELISA Kits
• CD31/PECAM-1 Lysate
• CD31/PECAM-1 Protein
• CD31/PECAM-1 Proteins and Enzymes
• CD31/PECAM-1 ELISAs