Why are there different molecular weight bands in western blot images for two of your antibodies (NBP1-84432 and H00010871-B01P) and overexpression lysate NBL1-08931?

Name: CD300c Antibody (03) [PE]
Brand: Novus Biologicals
SKU: NBP3-06451PE
Price: 369 USD

Question

Accepted Answer

A variety of band patterns can be expected when detecting CD300C. This protein has multiple glycosliation post-translational modifications that will change its molecular weight, and which vary in different experimental conditions. If you look at the uniprot link listed below, you can view more information on the specific PTMs for CD300C:https://www.uniprot.org/uniprot/Q08708#sequencesWe can be assured that these varying band patterns are indeed detection of CD300C by the absence of bands in the negative control. Any non-specific binding would show in both the negative and positive control, which is not the case here.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow
Clone	03
Clonality	Monoclonal
Host	Mouse
Conjugate	PE

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP3-06451AF350

Alexa Fluor 405 NBP3-06451AF405

Alexa Fluor 488 NBP3-06451AF488

Alexa Fluor 532 NBP3-06451AF532

Alexa Fluor 594 NBP3-06451AF594

Alexa Fluor 647 NBP3-06451AF647

Alexa Fluor 700 NBP3-06451AF700

Alexa Fluor 750 NBP3-06451AF750

Allophycocyanin NBP3-06451APC

Biotin NBP3-06451B

CoraFluor 1 NBP3-06451CL1

DyLight 350 NBP3-06451UV

DyLight 405 NBP3-06451V

DyLight 488 NBP3-06451G

DyLight 550 NBP3-06451R

DyLight 594 NBP3-06451DL594

DyLight 650 NBP3-06451C

DyLight 680 NBP3-06451FR

DyLight 755 NBP3-06451IR

FITC NBP3-06451F

HRP NBP3-06451H

Janelia Fluor 525 NBP3-06451JF525

Janelia Fluor 549 NBP3-06451JF549

Janelia Fluor 646 NBP3-06451JF646

Janelia Fluor 669 NBP3-06451JF669

PerCP NBP3-06451PCP

Unconjugated NBP3-06451-100ul

mFluor Violet 450 SE NBP3-06451MFV450

mFluor Violet 500 SE NBP3-06451MFV500

mFluor Violet 610 SE NBP3-06451MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NBP3-06451-100ul

Azide and BSA Free NBP3-06451-100ul

Azide and BSA Free NBP3-06451-50ul

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-06451AF350
Alexa Fluor 405	NBP3-06451AF405
Alexa Fluor 488	NBP3-06451AF488
Alexa Fluor 532	NBP3-06451AF532
Alexa Fluor 594	NBP3-06451AF594
Alexa Fluor 647	NBP3-06451AF647
Alexa Fluor 700	NBP3-06451AF700
Alexa Fluor 750	NBP3-06451AF750
Allophycocyanin	NBP3-06451APC
Biotin	NBP3-06451B
CoraFluor 1	NBP3-06451CL1
DyLight 350	NBP3-06451UV
DyLight 405	NBP3-06451V
DyLight 488	NBP3-06451G
DyLight 550	NBP3-06451R
DyLight 594	NBP3-06451DL594
DyLight 650	NBP3-06451C
DyLight 680	NBP3-06451FR
DyLight 755	NBP3-06451IR
FITC	NBP3-06451F
HRP	NBP3-06451H
Janelia Fluor 525	NBP3-06451JF525
Janelia Fluor 549	NBP3-06451JF549
Janelia Fluor 646	NBP3-06451JF646
Janelia Fluor 669	NBP3-06451JF669
PerCP	NBP3-06451PCP
Unconjugated	NBP3-06451-100ul
mFluor Violet 450 SE	NBP3-06451MFV450
mFluor Violet 500 SE	NBP3-06451MFV500
mFluor Violet 610 SE	NBP3-06451MFV610

CD300c Antibody (03) [PE]

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Bioinformatics

Immunogen	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD300c (Accession#: NP_006669.1; Met1-Arg183).
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	CD300C
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Flow Cytometry
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	PBS
Preservative	0.05% Sodium Azide
Purity	Protein A purified