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CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

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Western Blot: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.
Western Blot: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human prostate tissue.
Flow Cytometry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of K562 and Ramos cells, using anti-TNFRSF8 antibody (TA801630), (Red), compared to a nonspecific negative control antibody ...read more
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Adenocarcinoma of Human breast tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Carcinoma of Human bladder tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Carcinoma of Human kidney tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Carcinoma of Human thyroid tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human bladder tissue..
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human colon tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human endometrium tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human Kidney tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human lung tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human lymph node tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human lymphoma tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human Ovary tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human pancreas tissue.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human thyroid tissue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI1C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70374AF350
Alexa Fluor 405
NBP2-70374AF405
Alexa Fluor 488
NBP2-70374AF488
Alexa Fluor 532
NBP2-70374AF532
Alexa Fluor 594
NBP2-70374AF594
Alexa Fluor 647
NBP2-70374AF647
Alexa Fluor 700
NBP2-70374AF700
Alexa Fluor 750
NBP2-70374AF750
Allophycocyanin
NBP2-70374APC
Biotin
NBP2-70374B
CoraFluor 1
NBP2-70374CL1
DyLight 350
NBP2-70374UV
DyLight 405
NBP2-70374V
DyLight 488
NBP2-70374G
DyLight 550
NBP2-70374R
DyLight 594
NBP2-70374DL594
DyLight 650
NBP2-70374C
DyLight 680
NBP2-70374FR
DyLight 755
NBP2-70374IR
FITC
NBP2-70374F
HRP
NBP2-70374H
Janelia Fluor 525
NBP2-70374JF525
Janelia Fluor 549
NBP2-70374JF549
Janelia Fluor 646
NBP2-70374JF646
Janelia Fluor 669
NBP2-70374JF669
PE
NBP2-70374PE
PerCP
NBP2-70374PCP
Unconjugated
NBP2-46157
mFluor Violet 450 SE
NBP2-70374MFV450
mFluor Violet 500 SE
NBP2-70374MFV500
mFluor Violet 610 SE
NBP2-70374MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46157
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CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 19-379 of human TNFRSF8 (NP_001234) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
61.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

  • CD30 antigen
  • CD30
  • CD30KI-1
  • CD30L receptor
  • cytokine receptor CD30
  • D1S166EKi-1
  • Ki-1 antigen
  • Lymphocyte activation antigen CD30
  • TNFRSF8
  • tumor necrosis factor receptor superfamily member 8
  • tumor necrosis factor receptor superfamily, member 8

Background

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFRSF8