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CD23/Fc epsilon RII Antibody (SP23)

 (2 Publications)
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Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (SP23) [NB120-16702] - Formalin fixed paraffin embedded human tonsil stained with CD23 antibody (NB120-16702).
Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (SP23) [NB120-16702] - Formalin fixed paraffin embedded human tonsil stained with CD23 antibody (NB120-16702).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
SP23
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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CD23/Fc epsilon RII Antibody (SP23) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment, corresponding to amino acids 48-248 of Human CD23.
Localization
Cell Membrane
Specificity
This antibody recognizes CD23, a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells. It is strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukemia and some cases of centroblastic/centrocytic lymphoma.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FCER2
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Paraffin 1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30-60 min at RT.
Control
CD23/Fc epsilon RII Overexpression Lysate
Publications
Read Publications using
NB120-16702 in the following applications:

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD23/Fc epsilon RII Antibody (SP23)

  • BLAST-2
  • CD23
  • CD23A
  • CD23CD23 antigen
  • CLEC4J
  • CLEC4JC-type lectin domain family 4 member J
  • C-type lectin domain family 4, member J
  • Fc epsilon RII
  • Fc fragment of IgE, low affinity II, receptor for (CD23)
  • FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A)
  • fc-epsilon-RII
  • FCER2
  • Fcer2a
  • FceRII
  • IGEBF
  • Immunoglobulin E-binding factor
  • low affinity immunoglobulin epsilon Fc receptor
  • Ly-42
  • Lymphocyte IgE receptor

Background

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. CD23 is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD23/Fc epsilon
RII Antibody (SP23)
NB120-16702
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Publications for CD23/Fc epsilon RII Antibody (NB120-16702)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, Immunohistochemistry.


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Control Lysate(s)

CD23/Fc epsilon RII Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FCER2
Entrez