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CC2D1A Overexpression Lysate

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Western Blot: CC2D1A Overexpression Lysate (Adult Normal) [NBL1-08754] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CC2D1A.
Western Blot: CC2D1A Overexpression Lysate (Adult Normal) [NBL1-08754] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CC2D1A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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CC2D1A Overexpression Lysate Summary

Description

CC2D1A Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209105

Accession#: NM_017721

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CC2D1A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
103.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CC2D1A Overexpression Lysate

  • Akt kinase-interacting protein 1
  • coiled-coil and C2 domain containing 1A
  • coiled-coil and C2 domain-containing protein 1A
  • Five repressor element under dual repression-binding protein 1
  • FLJ20241
  • FLJ41160
  • FRE under dual repression-binding protein 1
  • FREUD-1
  • mental retardation, nonsyndromic, autosomal recessive, 3
  • MRT3Freud-1/Aki1
  • Putative NF-kappa-B-activating protein 023N
  • putative NFkB activating protein

Background

The CC2D1A gene encodes a transcriptional repressor that binds to a conserved 14-bp 5'-repressor element and regulates expression of the 5-hydroxytryptamine (serotonin) receptor 1A gene in neuronal cells. The DNA binding and transcriptional repressor activities

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CC2D1A